The use of species-specific primer targeting on D-loop mitochondrial for identification of wild boar meat in meatball formulation

Authors

  • Rien Larasati Arini
  • Dwiky Ramadhani
  • Ni wayan Pebriyanti
  • Sismindari Sismindari
  • Abdul Rohman

Keywords:

D-Loop 443 primer, Halal authentication, Meatball, Real-time PCR, Wild boar

Abstract

Objective: The study was intended to design the new specific primer targeting on mitochondrial D-Loop gene (D-Loop 443 primer) combined with a real-time polymerase chain reaction (PCR) for the analysis of wild boar meat (WBM) in food products of meatball.

Materials and methods: The primer was designed and subjected to primer-basic local alignment search tool using National Center for Biotechnology Information software. Validation of real-time PCR using designed primer was performed by evaluation of several performance characteristics which included specificity, sensitivity, repeatability, linearity, and efficiency.

Results: The results showed that the D-loop primer could be attached at 60.7°C and no amplification was detected against other species confirming the specificity of the primers. The limits of detection were found to be 4.68 ng and 2.34 ng using DNA extracted from WBM and that extracted from wild boar in meatball product. The D-Loop 443 primer was successfully used for the analysis of commercial meatball samples.

Conclusion: The developed method can be proposed as a standard method for the identification of WBM in meatball to support halal products authentication.

http://doi.org/10.5455/javar.2018.e275    

Downloads

Download data is not yet available.
Abstract
775
PDF
450

Author Biographies

Rien Larasati Arini

Faculty of Pharmacy, Gadjah Mada University, Sekip Utara, Yogyakarta 55281, Indonesia.

Dwiky Ramadhani

Faculty of Pharmacy, Gadjah Mada University, Sekip Utara, Yogyakarta 55281, Indonesia.

Ni wayan Pebriyanti

Faculty of Pharmacy, Gadjah Mada University, Sekip Utara, Yogyakarta 55281, Indonesia.

Sismindari Sismindari

Departement of Pharmaceutical Chemistry, Gadjah Mada University, Sekip Utara, Yogyakarta 55281, Indonesia.

Abdul Rohman

Departement of Pharmaceutical Chemistry, Gadjah Mada University, Sekip Utara, Yogyakarta 55281, Indonesia.

Downloads

Published

2018-10-02

How to Cite

Arini, R. L., Ramadhani, D., Pebriyanti, N. wayan, Sismindari, S., & Rohman, A. (2018). The use of species-specific primer targeting on D-loop mitochondrial for identification of wild boar meat in meatball formulation. Journal of Advanced Veterinary and Animal Research, 5(3), 361–368. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/38442

Issue

Vol. 5 No. 3 (2018)

Section

Short Communications

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).