The potential use of infrared spectroscopy and multivariate analysis for differentiation of beef meatball from dog meat for Halal authentication analysis

  • W.S. Rahayu
  • S. Martono
  • Abdul Rohman
Keywords: Bligh-Dryer, Chemometrics, Dog meat, Folch, FTIR spectroscopy

Abstract

Objective:  The objective of this research was to assess the suitability of FTIR spectroscopy coupled with multivariate analysis of partial least square regression (PLSR) along with pattern recognition technique of principal component analysis (PCA) for rapid quantitative and qualitative (identification) analysis of dog meat in beef meatball formulation.

Materials and Methods: The lipid fraction of meatball was obtained by employing two different extraction techniques, namely Bligh-Dyer and Folch method. FTIR spectral bands correlated with beef fat, pork fat, chicken fat and rat fat were measured, interpreted, and qualitatively analyzed. The small variations among spectra were exploited as a basis tools to differentiate between dog fat and other animal fats. 

Results: PCA at combined wavenumber regions of 1700-700 cm-1 was capable of identifying dog meat in meatball. These wavenumbers were also used for quantitative analysis of dog meat in meatball using PLSR model. Based on statistical parameters used, namely R2, RMSEC and RMSEP, Folch extraction method offered higher R2 and lower RMSEC and RMSEP than Bligh-Dyer. PCA is succesfully applied for classification between meatball containing dog meat and other meats.

Conclusion: FTIR spectroscopy coupled with multivariate analyses of PLSR and PCA was effective means for rapid screening of dog meat in meatball products.

http://doi.org/10.5455/javar.2018.e281

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Author Biographies

W.S. Rahayu

Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia.

 

Faculty of Pharmacy, Muhammadiyah University of Purwokerto. Purwokerto, Indonesia.

S. Martono
Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia.
Abdul Rohman

Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia.

 

Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia.

Published
2018-10-02
How to Cite
Rahayu, W., Martono, S., & Rohman, A. (2018). The potential use of infrared spectroscopy and multivariate analysis for differentiation of beef meatball from dog meat for Halal authentication analysis. Journal of Advanced Veterinary and Animal Research, 5(3), 307-314. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/38436
Section
Original Articles