Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo

  • Bwihangane Ahadi Birindwa
  • Gitao Chege George
  • Bisimwa Patrick Ntagereka
  • Okafor Christopher
  • Bebora Caroline Lilly
Keywords: Capripox, Coinfection, DRCongo, Goat, Phylogeny, PPRV

Abstract

Objective: We aimed at determining the prevalence and characterizing the CaPV, determining the CaPV-PPRV coinfection prevalence and providing data about phylogenetic relationship between the fusion protein of PPRV and P32 gene of CaPV.

Materials and methods:  A total of 150 samples including animals swabs, tissues and blood were collected from unvaccinated goats in a PPR and/or Capripox outbreaks in South Kivu, Eastern of Democratic Republic of the Congo. Conventional PCR and reverse transcriptase (RT-PCR) were used respectively to amplify P32, RPO30, GPCR genes of Capripox virus and Fusion (F) protein of PPRV. Positive samples were sequenced for phylogenetic analysis.

Results: Out of 150 tested animals, 64.7% (n=97/150) were PPRV positive, 52.7% (n=79/150) were Capripox positive and 38.7% (n=58/150) were positive for both PPRV and CaPV. The pairwise comparison of P32 gene of CaPV and F gene of PPRV showed 99.75% of identity percentage among goatpox virus sequences, 96.95% among PPRV sequences and 47.91% between CaPV and PPRV sequences.

Conclusion: The study has demonstrated high prevalence of CaP V-PPRV mixed infection in South Kivu.  Lumpy skin virus disease (LSVD) is a lineage circulating which has a genetic relationship between its P32 gene and the F gene of PPRV giving the challenge to differentiate the two diseases at the clinical farm level.

http://doi.org/10.5455/javar.2017.d233

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Author Biographies

Bwihangane Ahadi Birindwa

Department of Animal Science, Agriculture and Environmental faculty, Université Evangélique en Afrique , PO Box 3323 Bukavu, Democratic Republic of the Congo.

Department of Veterinary Pathology, Microbiology & Parasitology, Faculty of Veterinary Medicine, University of Nairobi, PO Box 29053-00625,Uthiru, Kenya.

 


 

Gitao Chege George

Department of Veterinary Pathology, Microbiology & Parasitology, Faculty of Veterinary Medicine, University of Nairobi, PO Box 29053-00625,Uthiru, Kenya.

Bisimwa Patrick Ntagereka

Department of Animal Science, Agriculture and Environmental faculty, Université Evangélique en Afrique , PO Box 3323 Bukavu, Democratic Republic of the Congo. 

Okafor Christopher

International Institute of Tropical Agriculture (IITA) Hub, Bukavu, Democratic Republic of the Congo.

Bebora Caroline Lilly

Department of Veterinary Pathology, Microbiology & Parasitology, Faculty of Veterinary Medicine, University of Nairobi, PO Box 29053-00625,Uthiru, Kenya.

Published
2018-01-29
How to Cite
Birindwa, B. A., George, G. C., Ntagereka, B. P., Christopher, O., & Lilly, B. C. (2018). Mixed infection of peste-des-petits ruminants and Capripox in goats in South Kivu, Democratic Republic of Congo. Journal of Advanced Veterinary and Animal Research, 4(4), 348-355. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/35374
Section
Original Articles