Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats

  • Tobias Nnia Egbe-Nwiyi
  • Ephraim Igwenagu
  • Anastasia Theresa Nwaosu
  • Meshach Maunta Maina
Keywords: Field isolate, Mixed infections, Pathogenicity, Parasitemia, Relapsed isolate

Abstract

Objective: This study was conceived to investigate the pathogenicity of relapsed (Diminazene aceturate-resistant), field (original) and mixed (relapsed and field) isolates of Trypanosoma brucei brucei in rats.

Materials and methods: Twenty eight healthy adult albino rats of both sexes weighing between 149-177 gm were used to compare the pathogenicity of relapsed, field and the mixed isolates of T. brucei brucei infections. The rats were separated into four groups (A-D); where, group A was kept as uninfected control, and group B was infected with 1x103 trypanosomes of the field isolate and 1x103 trypanosomes of the diminazene aceturate resistant isolate. The rats of groups C and D were infected with 1x106 trypanosomes of the diminazene aceturate-resistant isolate and 1x106 trypanosomes of the field isolate, respectively.

Results: The infected rats became parasitemic within 4 to 8 days post-infection. The mean pre-patent periods (PP) were 4.1±1.1, 6.0±2.0 and 9.1±1.1 days in groups B, C and D respectively, while the mean survival time (ST) in groups B, C and D were 21.4±10.1, 27.1±13.2 and 34.0 ±12.8 days, respectively. The PP and ST were shortest (P<0.05) in group B (mixed infections), and level of parasitemia was higher (P<0.05) in group B (mixed infections) as compared to groups C and D. The level of anemia was comparable (P>0.05) in groups C and D and more severe (P<0.05) in group B.

Conclusion: Mixed infections exhibit shortest PP, ST, higher level of parasitemia and more severe anemia, and appear to be more pathogenic.

http://doi.org/10.5455/javar.2017.d197             

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Author Biographies

Tobias Nnia Egbe-Nwiyi

Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria.

Ephraim Igwenagu

Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria.

Anastasia Theresa Nwaosu
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria.
Meshach Maunta Maina

Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria.

Published
2017-05-08
How to Cite
Egbe-Nwiyi, T. N., Igwenagu, E., Nwaosu, A. T., & Maina, M. M. (2017). Comparison of pathogenicity of relapsed, field and mixed isolates of Trypanosoma brucei brucei infections in rats. Journal of Advanced Veterinary and Animal Research, 4(1), 97-103. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/32477
Section
Original Articles