Para influenza virus 3 infection in cattle and small ruminants in Sudan

  • Intisar Kamil Saeed Virology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum, Sudan. #Current address: Northern Border University, Faculty of Science and Arts, Rafha
  • Yahia Hassan Ali Virology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum, Sudan. #Current address: Northern Border University, Faculty of Science and Arts, Rafha
  • Khalid Mohammed Taha Atbara Veterinary Research Laboratory, P.O. Box 121 Atbara, River Nile State
  • Nada ElAmin Mohammed Wad Medani Veterinary Research Laboratory, P.O. Box 230, Gezira State
  • Yasir Mehdi Nouri ElObied Veterinary Research Laboratory, P.O. Box 373, North Kordofan State
  • Baraa Ahmed Mohammed Virology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum, Sudan. #Current address: Northern Border University, Faculty of Science and Arts, Rafha
  • Osama Ishag Mohammed Rabak Veterinary Research Laboratory, P.O. Box 293 White Nile state
  • Salma Bushra Elmagboul Molecular Biology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum
  • Fahad AlTayeb AlGhazali Molecular Biology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum
Keywords: Cattle, Goats, PIV3, Pneumonia, Sheep, Sudan

Abstract

Objective: This study was aimed at elucidating the association between Para influenza virus 3 (PIV3) and respiratory infections in domestic ruminants in different areas of Sudan.

Materials and methods: During 2010-2013, five hundred sixty five lung samples with signs of pneumonia were collected from cattle (n=226), sheep (n=316) and goats (n=23) from slaughter houses in different areas in Sudan. The existence of PIV3 antigen was screened in the collected samples using ELISA and Fluorescent antibody technique. PIV3 genome was detected by PCR, and sequence analysis was conducted.

Results: Positive results were found in 29 (12.8%) cattle, 31 (9.8%) sheep and 11 (47.8%) goat samples. All the studied areas showed positive results. Highest prevalence (66.7%) was detected in the sheep and goats in Khartoum, followed by in goats in Nyala (33.3%) at western Sudan. Sequence analyses of PIV3 of different regions of Sudan indicated that these were similar in sequence and length. The BLAST analysis indicated that the test sequences were closely related to the available annotated sequences at the GenBank. All these sequences matched with Bovine parainfluenza virus 3 except two those were matching with Swine parainfluenza virus 3.

Conclusion: The results prove the existence of PIV3 infection in cattle, sheep and goats in the studied areas in Sudan and suggest its possible role in the respiratory infections. Genetic analysis indicate that the virus is mostly similar with bovine PIV3.

http://doi.org/10.5455/javar.2016.c160

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Author Biographies

Intisar Kamil Saeed, Virology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum, Sudan. #Current address: Northern Border University, Faculty of Science and Arts, Rafha

Yahia Hassan Ali, Virology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum, Sudan. #Current address: Northern Border University, Faculty of Science and Arts, Rafha

Khalid Mohammed Taha, Atbara Veterinary Research Laboratory, P.O. Box 121 Atbara, River Nile State

Nada ElAmin Mohammed, Wad Medani Veterinary Research Laboratory, P.O. Box 230, Gezira State

Yasir Mehdi Nouri, ElObied Veterinary Research Laboratory, P.O. Box 373, North Kordofan State

Baraa Ahmed Mohammed, Virology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum, Sudan. #Current address: Northern Border University, Faculty of Science and Arts, Rafha

Osama Ishag Mohammed, Rabak Veterinary Research Laboratory, P.O. Box 293 White Nile state

Salma Bushra Elmagboul, Molecular Biology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum

Fahad AlTayeb AlGhazali, Molecular Biology Department, Veterinary Research Institute, P.O. Box 8067, Al Amarat, Khartoum

Published
2016-11-03
How to Cite
Saeed, I. K., Ali, Y. H., Taha, K. M., Mohammed, N. E., Nouri, Y. M., Mohammed, B. A., Mohammed, O. I., Elmagboul, S. B., & AlGhazali, F. A. (2016). Para influenza virus 3 infection in cattle and small ruminants in Sudan. Journal of Advanced Veterinary and Animal Research, 3(3), 236-241. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/30216
Section
Original Articles