Detection of rabies in camel, goat and cattle in Sudan using Fluorescent antibody test (FAT) and hemi nested Polymerase Chain Reaction (hnRT-PCR)

  • Baraa Abdalaziz Ahmed Veterinary Resarch Institute, Khartoum
  • Yahia Hassan Ali Veterinary Resarch Institute, Khartoum
  • Omer Ahmed Veterinary Research Institute, Khartoum
  • Salma Elmagboul Veterinary Research Institute, Khartoum
  • Abdulghader Ballal Veterinary Research Institute, Khartoum and Veterinary Research Institute, PO Box 8067, Alamarat, Khartoum
Keywords: Camel, FAT, Rabies, hnRT-PCR

Abstract

Objective: The objective of this study was to identify rabies virus in camels and other animals in Sudan.

Materials and methods: Four camel samples were collected from Garraht Elzawia, Kab-kabia and North Darfur areas in Sudan. The samples were collected based on clinical signs. In addition, two camel samples were obtained from Khartoum and Tambool, one goat sample was collected from El-Fashir, and one cattle sample was obtained from Atbara. The samples were transported to the Veterinary Research Institute (VRI) at Khartoum, Sudan for further studies. The samples were subjected for nested and hemi nested RT-PCR (hnRT-PCR) along with the gold standard Fluorescent antibody test (FAT) to diagnose rabies.

Results: Out of eight samples, seven were found to be positive by both FAT and RT-PCR methods. The remaining one sample was positive by FAT but negative by hnRT-PCR indicating the suitablity of hnRT-PCR along with FAT for accurate diagnosis of rabies in animals.

Conclusion: The study concluded that FAT and RT-PCR are useful tools for research and diagnosis of rabies.

http://doi.org/10.5455/javar.2016.c162

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Author Biographies

Baraa Abdalaziz Ahmed, Veterinary Resarch Institute, Khartoum
Virology Department
Yahia Hassan Ali, Veterinary Resarch Institute, Khartoum
Virology Department
Omer Ahmed, Veterinary Research Institute, Khartoum
Virology Department
Salma Elmagboul, Veterinary Research Institute, Khartoum
Pathology Department
Abdulghader Ballal, Veterinary Research Institute, Khartoum and Veterinary Research Institute, PO Box 8067, Alamarat, Khartoum
Molecular Biology Department, and Viral Vaccines Production Department
Published
2016-11-03
How to Cite
Ahmed, B. A., Ali, Y. H., Ahmed, O., Elmagboul, S., & Ballal, A. (2016). Detection of rabies in camel, goat and cattle in Sudan using Fluorescent antibody test (FAT) and hemi nested Polymerase Chain Reaction (hnRT-PCR). Journal of Advanced Veterinary and Animal Research, 3(3), 274-277. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/30214
Section
Short Communications