Prevalence and phenotypic detection of methicillin resistance Staphylococcus aureus between ruminants butchered for humanoid intake and animal handlers in Maiduguri, Nigeria

  • Isa Adamu Gulani Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri, PMB 1069, Borno State
  • Yaqub Ahmed Geidam Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri, PMB 1069, Borno State
  • Lawan Adamu Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri, PMB 1069, Borno State
  • J. R. Lawal Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Maiduguri, PMB 1069, Borno State
  • Falmata Ali Abadam Department of Food Science and Technology, Ramat Polytechnic Maiduguri, PMB 1070, Borno State
Keywords: Human, Methicillin resistance, Ruminants, Staphylococcus aureus

Abstract

Objective: The objectives of this study was to investigate phenotypically the prevalence of Staphylococcus aureus and Methicillin Resistance S. aureus (MRSA) in ruminants and animal handlers in Maiduguri, Nigeria. 

Materials and methods: A total of 937 samples (900 nasal and skin swabs of cattle and 37 humans samples) were collected in Maiduguri metropolis. The samples were inoculated onto mannitol salt agar (MSA) and blood agar, and the cultrue plates were incubated at 37oC for 24 h. The S. aureus colonies showing golden yellow color on MSA were primarily identified as S. aureus, which were then subjected for catalase and coagulase tests. All S. aureus were finally screened for the presence of MRSA on oxalate resistant screening agar (ORSAB) medium. Antimicrobial susceptibility of the MRSA were measured by disc diffusion method.

Results: The overall prevalence of S. aureus was 44.3% (n=414/937). The MRSA could be detected in 12.1% (n=113/937) samples. Considerign different species, S. aureus could be isolated from 137 (45.6%), 148 (49.3%) and 119 (39.7%), 11 (29.7%) cattle, sheep, goat and humans, respectively. In ruminants, 27.7% (n-83/300) bulls were positive for S. aureus. Similarly, 10.7% (n=32/300) and 22.3% (n=67/300) were positive for S. aureus in rams and bucks, respectively. On the other hand, 18% (n=54/300), 38.7% (n=116/300) and 17.3% (n=52/300) samples from cows, ewes and does were positive for S. aureus. The highest MRSA could be isolated from ewes (9.3%; n=28/300) follwed by bulls (7%; n=21/300) and bucks (6.7%; n=20/300). In humans, 13.5% (n=5/37) samples were positive for MRSA.

Conclusion: S. aureus and MRSA infections in ruminants and animal handlers are documented in this study. The MRSA may exert public health threat to humans.

http://doi.org/10.5455/javar.2016.c145

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Abstract
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Published
2016-07-25
How to Cite
Gulani, I. A., Geidam, Y. A., Adamu, L., Lawal, J. R., & Abadam, F. A. (2016). Prevalence and phenotypic detection of methicillin resistance Staphylococcus aureus between ruminants butchered for humanoid intake and animal handlers in Maiduguri, Nigeria. Journal of Advanced Veterinary and Animal Research, 3(2), 152-159. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/28878
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Original Articles