Prevalence and antibiogram profile of Mycobacterium spp. in poultry and its environments

Authors

  • Md. Rubayet Reza Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Md. Bakhtiar Lijon Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Mst. Minara Khatun Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Md. Ariful Islam Department of Microbiology and Hygiene, FVS, BAU, Mymensingh

Keywords:

Antibiogram profile, Mycobacterium spp., Poultry farm, Polymerase chain reaction

Abstract

In this study, an attempt was undertaken to know the
prevalence and antibiogram profile of Mycobacterium
spp.  in  poultry  and  its  immediate  environments.  A
total of 130 samples comprising of droppings (n=80),
egg  washing  (n=18),  drinking  water  (n=14),  hand
washing  from  farm  workers  (n=6)  and  litter  (n=12)
were collected from six poultry farms located in and
around  Bangladesh  Agricultural  University  (BAU).
Samples  were  inoculated  onto  7H10  Middlebrook
agar and incubated aerobically at 37ºC for 7-14 days.
Identification of  Mycobacterium spp. was performed
by  colonial  morphology,  acid  fast  staining,  and
biochemical  tests.  Molecular  identification  of
Mycobacterium spp. at genus level was performed by
polymerase  chain  reaction  (PCR)  assay  targeting  65-
kDa heat shock protein gene. Antibiogram profile of
Mycobacterium  spp.  was  performed  against  five
antibiotics  namely  Rifampin,  Azithromycin,
Ciprofloxacin, Streptomycin and Doxycycline by disc
diffusion  method.  Three  Mycobacterium  spp.  were
isolated  from  dropping  samples  of  poultry.  The
overall  prevalence  of  Mycobacterium  spp.  was  2.3%
(n=3/130). All the isolates were resistant to Rifampin
and  sensitive  to  Azithromycin  and  Ciprofloxacin.
Data of this study indicated that multidrug resistant
Mycobacterium  spp.  are  prevalent  in  the  poultry
farms of the study area which underscore the need of
implementation  of  good  biosecurity  to  poultry
husbandry  practice  to  ensure  poultry  and  human
health.

http://dx.doi.org/10.5455/javar.2015.b118     

 

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Published

2015-12-31

How to Cite

Reza, M. R., Lijon, M. B., Khatun, M. M., & Islam, M. A. (2015). Prevalence and antibiogram profile of Mycobacterium spp. in poultry and its environments. Journal of Advanced Veterinary and Animal Research, 2(4), 458–463. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/26094

Issue

Vol. 2 No. 4 (2015)

Section

Original Articles

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