Molecular detection of Mycobacterium tuberculosis from bovine milk samples

  • V. BhanuRekha Department of Veterinary Public Health and Epidemiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry
  • L. Gunaseelan Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Ganesh Pawar Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, Chennai-07
  • Reza Nassiri International Institute of Health, Michigan State University, East Lansing, MI 48824
  • Sukumar Bharathy Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Tirupathi
Keywords: Bovine milk, Mycobacterium tuberculosis, Multiplex PCR, Reverse zoonoses

Abstract

Mycobacterium tuberculosis and Mycobacterium bovis are the major causes of tuberculosis. These may infect many animal species, and are likely to be the main source of infection in humans. A total of 181 bovine raw milk samples and 123 pre-scapular lymph node biopsy samples were collected and subjected to acid fast staining, fluorescent staining, isolation and identification. Genus specific PCR to identify the Mycobacterium tuberculosis complex (MTBC) organism, and multiplex PCR (mPCR) were done to differentiate M. tuberculosis and M. bovis. Among the milk samples tested, only one sample was culturepositive for M. tuberculosis. Four samples were positive by MTBC-PCR and mPCR; all these four were proved to be M. tuberculosis. It is quite likely that animals can be infected with human-originated M. tuberculosis, which in turn may act as a source of infection in humans, becoming a reverse zoonosis. Hence, control strategies for human tuberculosis caused by M. tuberculosis should necessarily include the control strategies in animals too.

http://dx.doi.org/10.5455/javar.2015.b44

 

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Abstract
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PDF
580
Published
2014-12-22
How to Cite
BhanuRekha, V., Gunaseelan, L., Pawar, G., Nassiri, R., & Bharathy, S. (2014). Molecular detection of Mycobacterium tuberculosis from bovine milk samples. Journal of Advanced Veterinary and Animal Research, 2(1), 80-83. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/21271
Section
Short Communications