Purification of bacteriocins using size-exclusion chromatography

Keywords: Bacteriocin, Size-exclusion chromatography

Abstract

The bacteriocin purification involves following main steps. a). Extraction of cell-free-supernatant of bacteria. b). Ammonium sulfate precipitation. c). Dialysis. d). Diafiltration using PVP and e). Size-exclusion chromatography. However, depending on the nature of work, the compound could be further analyzed by reverse-phase HPLC, NMR, mass spectrometry and sequencing.

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Part 1: 4 min 52 sec   Full Screen   Alternate

Part 2: 1 min 47 sec   Full Screen   Alternate

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Author Biographies

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Yeungnam University, Republic of Korea

Assistant Professor

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Irfan A. Rather, Department of Applied Microbiology and Biotechnology, Yeungnam University, Republic of Korea

Assistant Professor

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Alshammari Fanar Hamad, Department of Applied Microbiology and Biotechnology, Yeungnam University, Republic of Korea
Graduate Student

References

Ammonium sulfate calculator. EnCor Biotechnology Inc. 2015.

Gel filtration principles and methods. Amersham Biosciences. 18-1022-138.

Published
2016-03-13
How to Cite
Bajpai, V. K., I. A. Rather, and A. F. Hamad. “Purification of Bacteriocins Using Size-Exclusion Chromatography”. Bangladesh Journal of Pharmacology, Vol. 11, no. 2, Mar. 2016, pp. 281-4, doi:10.3329/bjp.v11i2.25862.
Section
Visual Experiment