Purification of bacteriocins using size-exclusion chromatography
Keywords:Bacteriocin, Size-exclusion chromatography
The bacteriocin purification involves following main steps. a). Extraction of cell-free-supernatant of bacteria. b). Ammonium sulfate precipitation. c). Dialysis. d). Diafiltration using PVP and e). Size-exclusion chromatography. However, depending on the nature of work, the compound could be further analyzed by reverse-phase HPLC, NMR, mass spectrometry and sequencing.
Video Clips of Methodology:
Part 1: 4 min 52 sec Full Screen Alternate
Part 2: 1 min 47 sec Full Screen Alternate
Ammonium sulfate calculator. EnCor Biotechnology Inc. 2015.
Gel filtration principles and methods. Amersham Biosciences. 18-1022-138.
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