Experimental strategy of animal trial for the approval of anti-diabetic agents prior to their use in pre-human clinical trials

Authors

DOI:

https://doi.org/10.3329/bjp.v11i1.25762

Keywords:

Blood glucose level, Diabetes induction, Diabetes mellitus, Experimental mice

Abstract

Although several naturally available drugs have been historically used for the treatment of diabetes mellitus throughout the world, few of them have been validated by scientific criteria. Before approval of any drug developed it should pass through animal trial prior to clinical human trial, which should followed by some standard ethical rules. Recently, a large diversity of animal models have been developed to better understand the pathogenesis of diabetes mellitus, and new drugs have been introduced in the market to treat this autoimmune disease. In the present article, we demonstrated some standard handling procedure of animal trial for the approval of anti-diabetic drug, which could be helpful for both academics and industrial scientific community to conduct the animal experiments. This research also contributes in the field of ethnopharmacology to design new strategies for the development of novel drugs to treat this serious condition of diabetes mellitus that constitutes a global public health.

Video Clip of Methodology

Handling and caring of mice: 2 min 30 sec   Full Screen   Alternate

Inducing diabetes in mice and observing blood glucose level: 1 min 47 sec   Full Screen   Alternate

Drug administration and observation of blood glucose level: 2 min 11 sec   Full Screen   Alternate

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Author Biographies

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Yeungnam University

Assistant Professor

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Irfan A. Rather, Department of Applied Microbiology and Biotechnology, Yeungnam University

Assitant Professor

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References

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Courteix C, Eschalier A, Lavarenne J. Streptozotocin-induced diabetic rats: Behavioral evidence for a model of chronic pain. Pain 1993; 53: 81-88.

Cunha JM, Funez MI, Cunha FQ, Parada CA, Ferreira SH. Streptozotocin induced mechanical hypernociception is not dependent on hyperglycemia. Braz J Med Biol Res. 2009; 42: 197-206.

Frode TS, Medeiros YS. Animal models to test drugs with potential anti-diabetic activity. J Ethnopharmacol. 2008; 115: 173-83.

Shukla S, Mehta P, Bajpai VK. Evaluation of comparative anti-diabetic effects of ethanolic extracts of Caesalpinia bouncucella and Stevia rebaudiana in normal and alloxan-induced experimental rats. Rom Biotechnol Lett. 2011; 16: 6187-99.

Soumyanath A. Traditional medicines for modern times: Anti-diabetic plants. London, CRC Press, 2006.

Turner MA. Screening methods in pharmacology. New York, Academic Press, 1965, p 26.

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Published

2015-12-15

How to Cite

Bajpai, V. K., I. A. Rather, and G.-J. Nam. “Experimental Strategy of Animal Trial for the Approval of Anti-Diabetic Agents Prior to Their Use in Pre-Human Clinical Trials”. Bangladesh Journal of Pharmacology, vol. 11, no. 1, Dec. 2015, pp. 30-34, doi:10.3329/bjp.v11i1.25762.

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Section

Visual Experiment