Evaluation of possible mechanisms of three plants for blood glucose control in diabetes

  • Damayanthi Dalu Department of Pharmacology, Netaji institute of Pharmaceutical Science, Toopranpet, Choutuppal, Nalgonda 508252, Telangana http://orcid.org/0000-0002-2407-822X
  • Satyavati Dhulipala Department of Pharmacology, Brilliant College of Pharmacy, Abdullapurmet, Hayatnagar, Ranga reddy, Hyderabad 501505,Telangana
Keywords: Cocculus orbiculatus, Diabetes, Leea indica, Ventilago maderaspatana

Abstract

This study was conducted to provide the evidence for the mechanism of  anti-diabetic activity of Cocculus orbiculatus, Leea indica and Ventilago maderaspatana. This was accomplished by employing methods like uptake of glucose, glycogen synthesis and inhibition of ?-glucosidase. For uptake of glucose, diaphragms were dissected out in Tyrode solution with 2% glucose and assayed for glucose content. In glycogen synthesis methodology liver, skeletal muscle and cardiac muscles were isolated, homogenized and glycogen content was analyzed. In ?-glucosidase enzyme inhibition procedure involved estimation of ?-glucosidase enzyme inhibition. All the three plant extracts exhibited significant (p<0.05 - p<0.01) anti-diabetic activity by increasing glucose uptake, glycogen synthesis and inhibiting ?-glucosidase enzyme. Among the three plants, V. maderaspatana (500 mg/kg) exhibited higher glucose uptake, glycogen content and ?-glucosidase inhibition activity (IC50 145 µg/mL). The present experimental results evidenced the anti-diabetic activity of three plants by all the three mechanisms.

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Author Biographies

Damayanthi Dalu, Department of Pharmacology, Netaji institute of Pharmaceutical Science, Toopranpet, Choutuppal, Nalgonda 508252, Telangana
Assosciate Professor
Satyavati Dhulipala, Department of Pharmacology, Brilliant College of Pharmacy, Abdullapurmet, Hayatnagar, Ranga reddy, Hyderabad 501505,Telangana
Professor

References

Barham D, Trinder P. An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst 1972. 97: 142-45.

Carroll NV, Longely RW, Rose JH. The determination of glycogen in liver and muscle by use of anthrone reagent. J Biol Chem. 1956; 220: 583-93.

Chattopadhyay RR, Sarkar SK, Ganguly S, Banerjee RN, Basu TK. Effect of leaves of Vinca rosea Linn. on glucose utilization and glycogen deposition by isolated rat hemidiaphragm. Indian J Physiol Pharmacol. 1992; 36: 137-38.

Cheng Z, Pang T, Gu M, Gao AH, Xie CM, Li JY, Nan FJ, Li J. Berberine-stimulated glucose uptake in L6 myotubes involves both AMPK and p38 MAPK. Biochim Biophys Acta. 2006; 1760: 1682-89.

Damayanthi D, Satyavati D, Suresh A. Antihyperglycemic and hypolipidemic activity of Leea indica. Int J Bioassays. 2014; 3: 3155-59.

Damayanthi D, Satyavati D. Anti-diabetic and anti-hyperlipidemic activity of alcoholic and hydroalcoholic extracts of Cocculus orbiculatus in streptozotocin induced diabetic rats. Am J Phytomed Clin Ther. 2015; 3: 276-86.

Damayanthi D, Satyavati D. Anti-diabetic, anti-hyperlipidemic and anti-oxidant properties of roots of Ventilago maderaspatana Gaertn. on streptozotocin-induced diabetic rats. IOSR: J Pharm Biol Sci. 2015; 10: 50-59.

Harbone JB. Phytochemical methods: A guide to modern techniques of plant analysis. 3rd ed. New York, Chapman and Hall Publisher, Springer, 1998, p 302.

Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI. Inhibitory effect of pine extract on ?-glucosidase activity and postprandial hyperglycemia. Nutrition 2005; 21: 756-61.

Kim SH, Shin EJ, Kim ED, Bayaraa T, Frost SC, Hyun CK. Berberine activates GLUT1- mediated glucose uptake in 3T3-L1 adipocyte. Biol Pharm Bull. 2007; 30: 2120-25.

Klip A, Leiter L.A. Cellular mechanism of action of metformin. Diabetes Care. 1990; 13: 696-704.

Ko BS, Choi SB, Park SK, jang JS, Kim YE, Park S. Insulin sensitizing and insulinotropic action of berberine from Cortidis rhizome. Biol Pharm Bull. 2005; 28: 1431-37.

Krentz AJ, Bailey CJ. Oral antidiabetic agents: Current role in type 2 diabetes mellitus. Drugs 2005; 65: 385-411.

Kuppusamy A, Muthusamy U, Andichetiar Thirumalaisamy S, Varadharajan S, Ramasamy K, Ramanathan S. In vitro (?-glucosidase and ?amylase inhibition) and in vivo antidia-betic property of phytic acid (IP 6) in streptozotocin-nicotinamide induced type 2 diabetes mellitus (NIDDM) in rats. J Complement Integrat Med. 2011; 8.

Lee YS, Kim WS, Kim KH, Yoon MJ, Cho HJ, Shen Y, Ye JM, Lee CH, Oh WK, Kim CT. Berberine a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states. Diabetes 2006; 55: 2256-64.

Pan GY, Huang ZJ, Wang GJ, Fawcett JP, Liu XD, Zhao XC, Sun JG, Xie YY. The anti-hyperglycaemic activity of berberine arises from a decrease of glucose absorption. Planta Med. 2003; 69: 632-36.

Punithavathi VR, Stanely Mainzen Prince P, Kumar MR, Selva Kumara CJ. Protective effects of gallic acid on hepatic lipid peroxide metabolism, glycoprotein components and lipids in streptozotocin-induced type II diabetic Wistar rats. J Biochem Molecular Toxic. 2011; 25: 68-76.

Shulman GI. Cellular mechanisms of insulin resistance. J Clin Invest. 2000; 106: 171-76.

Sunil Kumar, Vipin Kumar, Om Prakash. Enzymes inhibition and Anti-diabetic effect of isolated constituents from Dillenia indica. Biomed Res Int. 2013; 2013: 1155-62.

Vishnu Prasad CN, Anjana T, Banerji A, Gopalakrishnapillai A. Gallic acid induces GLUT 4 translocation and glucose uptake activity in 3T3 cells. FEBS Lett. 2010; 584: 531-36.

Walass E, Walass O. Effect of insulin on rat diaphragm under anaerobic conditions. J Biol Cem. 1952; 195: 367-73.

Wang SH, Wang WJ, Wang XF, Chen W. Effect of Astragalus polysaccharides and berberine on carbohydrate metabolism and cell differentiation in 3T3-L1 adipocytes. Chin J Integr Tradit Chin West Med. 2004; 24: 926-28.

Yang D, Zhao J, Liu S, Song F, Liu ZQ. The screening of potential ?-glucosidase inhibitors from the Polygonum multiflorum extract using ultra-filtration combined with liquid chromatography-tandem mass spectrometry. Analyl Methods. 2014; 6: 3353-59.

Yang Y, Shang W, Zhou L, Jiang B, Jin H, Chen M. Emodin with PPAR? ligand-binding activity promotes adipocyte differentiation and increases glucose uptake in 3T3-L1 cells. Biochem Biophyl Res Comm. 2007; 353: 225-30.

Yin J, Gao Z, Liu D, Liu Z, Ye J. Berberine improves glucose metabolism through induction of glycolysis. Am J Physiol Endocrinol Metab. 2008; 294: E148-56.

Yin J, Hu R, Chen M, Tang J, Li F, Yang Y, Chen J. Effects of berberine on glucose metabolism in vitro. Metabolism 2002; 51: 1439-43.

Yonemitsu S, Nishimura H, Shintani M, Yoshihiro O, Tatsuya H, Kiminori H, Inoue G, Kazuwa N. Troglitazone induces GLUT 4 translocation in L6 myotubes. Diabetes 2001; 50: 1093-101.

Zhou LB, Chen MD, Song HD, Yang Y, Wang X, Tang JF, Li FY, Xu MY, Chen JL. Effect of berberine on glucose transport in adipocytes and its mechanism. Chin J Endocrinol Metab. 2003; 19: 479-82.

Zhou LB, Chen MD, Wang X, Song HD, Yang Y, Tang JF, Li FY, Xu MY, Chen JL. Effect of berberine on the differentiation of adipocytes. Zhonghua Yi Xue Za Zhi. 2003; 83: 338-40.

Zhou L, Yang Y, Wang X, Liu S, Shang W, Yuan G, Li F, Tang J, Chen M, Chen J. Berberine stimulates glucose transport through a mechanism distinct from insulin. Metabolism 2007; 56: 405-12.

Published
2016-01-23
How to Cite
Dalu, D., and S. Dhulipala. “Evaluation of Possible Mechanisms of Three Plants for Blood Glucose Control in Diabetes”. Bangladesh Journal of Pharmacology, Vol. 11, no. 1, Jan. 2016, pp. 224-30, doi:10.3329/bjp.v11i1.24932.
Section
Research Articles