Antibacterial activity of seneciolactone isolated from Senecio scandens against some common gastrointestinal tract disease causing bacterial pathogens
Keywords:Antibacterial, Bacterial pathogen, Gastrointestinal tract, Senecio scandens, Seneciolactone
The objective of the present investigation was to study the antibacterial effect of seneciolactone isolated from the methanolic extract of Senecio scandens against five bacterial pathogens which are known to cause several gastrointestinal tract diseases. Disc diffusion assay and agar well diffusion assays were used to examine the antibacterial efficacy of this compound by measuring zones of inhibition and MIC/MBC values. Scanning electron microscopy (SEM) was involved to study the effect of this compound on cellular morphology of Shigella dysenteriae. Results revealed that seneciolactone exhibited moderate to potent antibacterial activity against different bacterial strains. Zones of inhibition and MIC/MBC values indicated that seneciolactone was most potent against S. dysenteriae followed by Pseudomonas aeruginosa and E. coli. SEM results indicated that seneciolactone induced potent damage to the cell membrane of the tested bacteria. As compared to the untreated control which exhibited normal cellular morphology, the seneciolactone treated bacterial cells revealed severe damage to the cellular membrane particularly at the higher doses.
Agizzio AP, Da Cunha M, Carvalho AO, Oliveira MA, Ribeiro SFF, Gomes VM. The antifungal properties of a 2S albumin-homologous protein from passion fruit seeds involve plasma membrane permeabilization and ultra structural alterations in yeast cells. Plant Sci. 2006; 17: 515-22.
Balandrin MF, Klocke JA, Wutule ES, Bollinger WH. Natural plant chemicals: Sources of industrial and medicinal materials. Science 1985; 228: 115460.
Batra V, Rajagopalan TR. Alkaloidal constituents of Senecio scandens. Curr Sci. 1977; 46: 141.
Bohlmann F, Knoll KH, Zdero C, Mahanta PK, Greaz M, Suwita A, Ehlera A, Le Van N, Abraham WR, Natu AA. Terpen-derivate aus Senecioarten. Phytochemistry 1977; 16: 965-85.
Brito SM, Coutinho HD, Talvani A, Coronel C, Barbosa AG, Vega C, Figueredo FG, Tintino SR, Lima LF, Boligon AA, Athayde ML, Menezes IR. Analysis of bioactivities and chemical composition of Ziziphus joazeiro Mart. using HPLC-DAD. Food Chem. 2015; 186: 185-91.
Cabral V, Luo X, Junqueira E, Costa SS, Mulhovo S, Duarte A, Couto I, Viveiros M, Ferreira MJ. Enhancing activity of antibiotics against Staphylococcus aureus: Zanthoxylum capense constituents and derivatives. Phytomedicine. 2015; 15; 22: 469-76.
Djouossi MG, Tamokou JD, Ngnokam D, Kuiate JR, Tapondjou LA, Harakat D, Voutquenne-Nazabadioko L. Antimicrobial and anti-oxidant flavonoids from the leaves of Oncoba spinosa Forssk. (Salicaceae). BMC Complement Altern Med. 2015; 28; 15: 134.
Harrison JW, Svec TA. The beginning of the end of the antibiotic era? Part II. Proposed solutions to antibiotic abuse. Quintessence Int. 1998; 29: 22329.
Jones FA. Herbs-useful plants. Their role in history and today. Eur J Gastroenterology Hepatol. 1996; 8: 122731.
Mota AS, Martins MR, Arantes S, Lopes VR, Bettencourt E, Pombal S, Gomes AC, Silva LA. Antimicrobial activity and chemical composition of the essential oils of Portuguese Foeniculum vulgare fruits. Nat Prod Commun. 2015; 10: 673-76.
Rao GG. Risk factors for the spread of antibiotic-resistant bacteria. Drugs. 1998; 55: 323-30.
Satish S, Raveesha KA, Janardhana GR. Antibacterial activity of plant extracts of phytopathogenic Xanthomonas campestris pathovars. Lett Appl Microbiol. 1999; 28: 145147.
Tian XY, Wang YH, Yang QY, Liu X, Fang WS, Yu SS. Jacaranone glycosides from Senecio scandens. J Asian Nat Prod Res. 2006; 8: 125-32.
Wang D, Huang L, Chen S. Senecio scandens Buch.-Ham.: A review on its ethnopharmacology, phytochemistry, pharmacology, and toxicity. J Ethnopharmacol. 2013; 149: 123.
Wang XF, Tu DJ. Studies on the chemical constituents of Senecio scandens Buch,-Ham (author's transl). Acta Pharm Sin. 1980; 15: 503-05.
Weisser R, Asscher AW, Winpenny J. In vitro reversal of antibiotic resistance by ethylenediamine tetraacetic acid. Nature 1996; 219: 1365-66.
Yu J, Lei J, Yu H, Cai X, Zou G. Chemical composition and antimicrobial activity of the essential oil of Scutellaria barbata. Phytochemistry 2004; 65: 881-84.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).