Acetoxyroyleanone exhibits selective anti-cancer effects and induces apoptosis in human colon carcinoma cells through the mediation of NF-kappaB and caspase-3 signalling pathways

Authors

  • Xiao-Jun Zhong Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province
  • Hui Jia Departments of Internal Medicine-Oncology?Shandong Cancer Hospital and Institute, Jinan 250117, Shandong Province
  • Ji-Long Hu Department of Tumor Abdominal Surgery, Jiangxi Tumor Hospital, Nanchang 330029, Jiangxi Province,
  • Fei Le Department of Head and Neck Surgery, Jiangxi Province Tumor Hospital, Nanchang 330029, Jiangxi Province,
  • Xiao-Ming Zhong Department of Tumor Radiotherapy and Chemotherapy, Jiangxi Tumor Hospital, Nanchang 330029, Jiangxi Province http://orcid.org/0000-0002-1027-7316

DOI:

https://doi.org/10.3329/bjp.v11i1.24339

Keywords:

Acetoxyroyleanone, Anti-cancer, Apoptosis, Caspase-3, Human colon carcinoma cell, NF-?B

Abstract

The objective of the current study was to evaluate the antiproliferative and apoptotic activities of acetoxyroyleanone against various cancer cells along with studying its effect on chromatin condensation, NF-?B and caspase-3 expressions and cell migration. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was used to evaluate cell viability while as fluorescence microscopy revealed the effects of acetoxyroyleanone  on cellular morphology of Colo-205 cells. Western blotting revealed effects on NF-?B and caspase-3 expressions. The results revealed that acetoxyroyleanone  induced potent and dose-dependent antiproliferative effects against a range of cancer cell lines with Colo-205 being the most susceptible cell line. However, it required six to eight times higher concentration of acetoxyroyleanone to induce 50% cell death in normal epithelial (fR-2) cell line. Further, following acetoxyroyleanone treatment to Colo-205, it was observed that acetoxyroyleanone induced substantial down-regulation of NF-?B and up-regulation of caspase-3 expressions. In addition, acetoxyroyleanone impairs cell migration, chromatin condensation, cell shrinkage and membrane blebbing.

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Author Biographies

Xiao-Jun Zhong, Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province

Associate Professor

Hui Jia, Departments of Internal Medicine-Oncology?Shandong Cancer Hospital and Institute, Jinan 250117, Shandong Province

Associate Professor

Ji-Long Hu, Department of Tumor Abdominal Surgery, Jiangxi Tumor Hospital, Nanchang 330029, Jiangxi Province,

Associate Professor

Fei Le, Department of Head and Neck Surgery, Jiangxi Province Tumor Hospital, Nanchang 330029, Jiangxi Province,

Associate Professor

Xiao-Ming Zhong, Department of Tumor Radiotherapy and Chemotherapy, Jiangxi Tumor Hospital, Nanchang 330029, Jiangxi Province

Associate Professor

 

Published

2016-01-01

How to Cite

Zhong, X.-J., H. Jia, J.-L. Hu, F. Le, and X.-M. Zhong. “Acetoxyroyleanone Exhibits Selective Anti-Cancer Effects and Induces Apoptosis in Human Colon Carcinoma Cells through the Mediation of NF-KappaB and Caspase-3 Signalling Pathways”. Bangladesh Journal of Pharmacology, vol. 11, no. 1, Jan. 2016, pp. 141-7, doi:10.3329/bjp.v11i1.24339.

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Section

Research Articles