Proposed mechanism of antibacterial mode of action of Caesalpinia bonducella seed oil against food-borne pathogens
Keywords:Antibacterial, Caesalpinia bonducell, Food-borne pathogen
The antibacterial mechanism of action of Caesalpinia bonducella seed oil on membrane permeability of Listeria monocytogenes NCIM 24563 (MIC: 2 mg/mL) and Escherichia coli ATCC 25922 (MIC: 4 mg/mL) was determined by measuring the extracellular ATP concentration, release of 260-nm absorbing materials, leakage of potassium ions and measurement of relative electrical conductivity of the bacterial cells treated at MIC concentration. Its mode of action on membrane integrity was confirmed by release of extracellular ATP (1.42 and 1.33 pg/mL), loss of 260-nm absorbing materials (4.36 and 4.19 optical density), leakage of potassium ions (950 and 1000 mmol/L) and increase in relative electrical conductivity (12.6 and 10.5%) against food-borne pathogenic bacteria L. monocytogenes and E. coli, respectively. These findings propose that C. bonducella oil compromised its mode of action on membrane integrity, suggesting its enormous food and pharmacological potential.
Aiyelaagbe OO, Adesoganm K, Ekundayo O, Gloer JB. Antibacterial diterpenoids from Jatropha podagrica Hook. Phytochemistry. 2007; 68: 2420-25.
Al-Reza SM, Rahman A, Lee JH, Kang SC. Potential roles of essential oil and organic extracts of Zizyphus jujube in inhibiting food-borne pathogens. Food Chem. 2010; 119: 981-86.
Ashebir M, Ashenafi M. Assessment of the antibacterial activity of some traditional medicinal plants on some food-borne pathogens. Ethiop J Health Dev. 1999; 13: 211-16.
Bajpai VK, Sharma A, Baek KH. Antibacterial mode of action of Cudrania tricuspidata fruit essential oil, affecting membrane permeability and surface characteristics of food-borne pathogens. Food Cont. 2013; 32: 582-90.
Carson CF, Mee BJ, Riley TV. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy. Antimicrob Agents Chemother. 2002; 46: 1914-20.
Chakrabarti S, Biswas TK, Rokeya B, Ali L, Mosihuzzaman M, Nahar N, Khan AKA, Mukherjee B. Advanced studies on the hypoglycemic effect of Caesalpinia bonducella F. in type 1 and 2 diabetes in long Evans rats. J Ethnopharmacol. 2003; 84: 41-46.
Cox SD, Gustafson JE, Mann CM, Markhan JL, Liew YC, Hartlnd RP. Tea tree oil causes Kþ leakage and inhibits respiration in Escherichia coli. Lett Appl Microbiol. 1998; 26: 355-58.
Cox SD, Mann CM, Markhan JL, Gustafson JE, Warmington JR, Wyllie SG. Determining the antimicrobial action of tea tree oil. Molecules 2001; 6: 87-91.
Denyer SP. Mechanisms of action of biocides. Int Biodeter. 1990; 26: 89-100.
Farag RS, Daw ZY, Hewedi FM, El-Baroty GSA. Antimicrobial activity of some Egyptian spice essential oil. J Food Prot. 1989; 52: 665-67.
Giang PM, Son PT, Matsunami K, Otsuka H. Anti-staphylococcal activity of ent-kaurane-type diterpenoids from Croton tonkinensis. J Nat Med. 2006; 60: 93-95.
Helander IM, Alakomi HL, Latva-Kala K, Mattila-Sandholm T, Pol I, Smid EJ. Characterization of the action of selected essential oil components on Gram-negative bacteria. J Agric Food Chem. 1998; 46: 3590-95.
Herranz C, Chen Y, Chung HJ, Cintas LM, Hernandez PE, Montville TJ, Chikindas ML. Enterocin P selectively dissipates the membrane potential of Enterococcus faecium T136. Appl Environ Microbiol. 2001; 67: 1689-92.
Patra JK, Hwang H, Choi JW, Baek KH. Bacterial mechanism of bio-oil obtained from fast pyrolysis of Pinus densiflora against two food-borne pathogens, Bacillus cereus and Listeria monocytogenes. Foodborne Pathogens Dis. 2015; 12: 529-35.
Rahman A, Kang SC. In vitro control of food-borne and food spoilage bacteria by essential oil and ethanol extracts of Lonicera japonica Thunb. Food Chem. 2010; 116: 670-75.
Shukla S, Mehta A, Mehta P, Vyas SP, Shukla S, Bajpai VK. Studies on anti-inflammatory, antipyretic and analgesic properties of Caesalpinia bonducella F. seed oil in experimental animal models. Food Chem Toxicol. 2010; 48: 61-64.
Shukla S, Mehta A, Agrawal P. Antibacterial and anti-lipid peroxidation inhibitory effects of Caesalpinia bonducella seed oil. J Essen Oil Bear Plant. 2013 (In press).
Sultana R, Saleem R, Sultana N, Afshan F, Gulzar T. Characterization of the composition of Caesalpinia bonducella seed grown in temperate regions of Pakistan. J Am Oil Chem Soc. 2012; 89: 1021-27.
Tauxe VR. Emerging foodborne pathogens. Int J Food Microbiol. 2002; 78: 31-34.
Yoon JI, Bajpai VK, Kang SC. Synergistic effect of nisin and cone essential oil of Metasequoia glyptostroboides Miki ex Hu against Listeria monocytogenes in milk samples. Food Chem Toxicol. 2011; 49: 109-14.
Zurenko GE, Yagi BH, Schaadt RD, Allison JW, Kilburn JO, Glickman SE, Hutchinson DK, Barbachyn MR, BricknerSJ. In vitro activities of U-100592 and U-100766, novel oxazolidinone antibacterial agents. Antimicrob Agents Chemother. 1996; 40: 839-45.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).