In silico and antithrombotic studies of 1,3,4-oxadiazoles derived from benzimidazole

Authors

  • B. Vishwanathan Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Sri Shivarathreeshwara Nagar, Mysore, Karnataka 570015 http://orcid.org/0000-0003-0924-3466
  • B. M. Gurupadayya Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Sri Shivarathreeshwara Nagar, Mysore, Karnataka 570015
  • K. Venkata Sairam Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Sri Shivarathreeshwara Nagar, Mysore, Karnataka 570015

DOI:

https://doi.org/10.3329/bjp.v11i1.23981

Keywords:

Antithrombotic, Benzimidazole, In silico, Oxadiazole

Abstract

In the present study, a series of 1,3,4-oxadiazole derivatives (4a-4k) derived from benzimidazole were docked onto factor Xa (PDB: 1NFY) protein using SYBYLX 2.1. and also evaluated for in vitro clot lysis for thrombolytic activity. The synthesized molecules were also screened for in silico ADME studies. The molecular docking studies highlighted that the molecules showed high affinity towards 1NFY with higher docking score and the in silico ADME results were promising and indicated that the molecules holds great potential as a drug candidate. The thrombolytic evaluation was performed for decrease in solid clot weight by the clot lysis study at a concentration of 6.25, 12.5 and 25 µM strengths, respectively. The results of in vitro clot lysis for thrombolytic evaluation revealed that the tested compounds 4a-4k exhibited significant clot lysis with respect to negative control phosphate buffered saline and in comparison to the reference drug streptokinase (30,000 IU). Among all the tested compounds, compound 4j, 4d and 4g exhibited potent thrombolytic activity with EC50 value of 16.2, 18.1 and 23.7 µM, respectively. The thrombolytic efficacy investigation highlights that the synthesized compound 4j could be considered for further clinical studies to ascertain its possible hit as thrombolytic agents.

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Author Biography

B. Vishwanathan, Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Sri Shivarathreeshwara Nagar, Mysore, Karnataka 570015

Lecturer

Published

2015-12-18

How to Cite

Vishwanathan, B., B. M. Gurupadayya, and K. V. Sairam. “In Silico and Antithrombotic Studies of 1,3,4-Oxadiazoles Derived from Benzimidazole”. Bangladesh Journal of Pharmacology, vol. 11, no. 1, Dec. 2015, pp. 67-74, doi:10.3329/bjp.v11i1.23981.

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Section

Research Articles