5-Bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran-2-one targets prostate cancer cells by down-regulating inflammation-related genes

Authors

  • Zhao-Yang Wang Department of Urology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007
  • Jun-Yong Wang Department of Urology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007
  • Jun Zhang Department of Urology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007
  • Kai Zhang Department of Urology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007
  • Rui Fan Department of Urology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007
  • Jun-Hao Niu Department of Urology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007 http://orcid.org/0000-0002-5988-5646

DOI:

https://doi.org/10.3329/bjp.v11i2.23872

Keywords:

Cancer cell, Inflammation-related gene, Prostate

Abstract

The present study was performed to examine the effect of 5-bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran-2-one (BHP) on the rate of cell proliferation and apoptosis induction in the PC3, human prostate carcinoma cell line. The cell viability was assayed by using sulphorhodamine B staining and apoptosis by annexin V and flow cytometry analyses. The results revealed that BHP treatment in PC3 cells caused a significant reduction in the rate of cell proliferation in dose- and time-dependent manner. Compared to the un-treated cells, the formation of HUVEC tubes was markedly inhibited on treatment with BHP at a concentration of 30 µM. Further investigation revealed the expression of HMGB1, IL-6 and IL-8, pro-inflammatory cytokines was also inhibited on treatment with BHP. Therefore, BHP treatment plays an important role in inducing apoptosis in the prostrate cells and can be of therapeutic value for the prostate cancer treatment.

Downloads

Download data is not yet available.
Abstract
1334
Download
748 Read
528

References

Coussens LM, Werb Z. Inflammation and cancer. Nature 2002; 420: 860-67.

Dickinson JM. Microbial pyran-2-ones and dihydropyran-2-ones. Nat Prod Rep. 1993; 10: 71-98.

Faulkner DJ. Marine natural products. Nat Prod Rep. 2001; 18: 1-49.

Gnanasekar M, Thirugnanam S, Ramaswamy K. Short hairpin RNA (shRNA) constructs targeting high mobility group box-1 (HMGB1) expression leads to inhibition of prostate cancer cell survival and apoptosis. Int J Oncol. 2009; 34: 425-31.

Jemal A, Siegel R, Xu J, Ward E. Cancer statistics, 2010. CA Cancer J Clin. 2010; 60: 277-300.

Jensen PR, Fenical W. Strategies for the discovery of secondary metabolites from marine bacteria: Ecological perspectives. Annu Rev Microbiol. 1994; 48: 559-84.

Lu H, Ouyang W, Huang C. Inflammation, a key event in cancer development. Mol Cancer Res. 2006; 4: 221-33.

McGlacken GP, Fairlamb IJ. 2-Pyrone natural products and mimetics: Isolation, characterisation and biological activity. Nat Prod Rep. 2005; 22: 369-85.

Pfitzenmaier J, Vessella R, Higano CS, Noteboom JL, Wallace D Jr, Corey E. Elevation of cytokine levels in cachectic patients with prostate carcinoma. Cancer 2003; 97: 1211-16.

Poppe SM, Slade DE, Chong KT, Hinshaw RR, Pagano PJ, Markowitz M, Ho DD, Mo H, Gorman RR 3rd, Dueweke TJ, et al., Antiviral activity of the dihydropyrone PNU-140690, a new non-peptidic human immunodeficiency virus protease inhibitor. Antimicrob Agents Chemother. 1997; 41: 1058-63.

Thaisrivongs S, Romero DL, Tommasi RA, Janakiraman MN, Strohbach JW, Turner SR, Biles C, Morge RR, Johnson PD, Aristoff PA, et al., Structure-based design of HIV protease inhibitors: 5,6-dihydro-4-hydroxy-2-pyrones as effective, non-peptidic inhibitors. J Med Chem. 1996; 39: 4630-42.

Trachootham D, Lu W, Ogasawara MA, Nilsa RD, Huang P. Redox regulation of cell survival. Antioxid Redox Signal. 2008; 10: 1343-74.

Turner SR, Strohbach JW, Tommasi RA, Aristoff PA, Johnson PD, Skulnick HI, Dolak LA, Seest EP, Tomich PK, Bohanon MJ, et al., Tipranavir (PNU-140690): A potent, orally bioavailable non-peptidic HIV protease inhibitor of the 5,6-dihydro-4-hydroxy-2-pyrone sulfonamide class. J Med Chem. 1998; 41: 3467-76.

Uehara H, Troncoso P, Johnston D, et al. Expression of interleukin-8 gene in radical prostatectomy specimens is associated with advanced pathologic stage. Prostate 2005; 64: 40-49.

Veltri RW, Miller MC, Zhao G, et al., Interleukin-8 serum levels in patients with benign prostatic hyperplasia and prostate cancer. Urology 1999; 53: 139-47.

Wakui S, Furusato M, Itoh T, et al. Tumour angiogenesis in prostatic carcinoma with and without bone marrow metastasis: A morphometric study. J Pathol. 1992; 168: 257-62.

Weidner N, Carroll PR, Flax J, Blumenfeld W, Folkman J. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma. Am J Pathol. 1993; 143: 401-09.

Downloads

Additional Files

Published

2016-03-26

How to Cite

Wang, Z.-Y., J.-Y. Wang, J. Zhang, K. Zhang, R. Fan, and J.-H. Niu. “5-Bromo-3-(3-Hydroxyprop-1-Ynyl)-2H-Pyran-2-One Targets Prostate Cancer Cells by down-Regulating Inflammation-Related Genes”. Bangladesh Journal of Pharmacology, vol. 11, no. 2, Mar. 2016, pp. 408-13, doi:10.3329/bjp.v11i2.23872.

Issue

Vol. 11 No. 2 (2016)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).