Lower temperature (27°C) Decreased Total Motile Sperm Count but not DNA Fragmentation Index in Sperm Preparation with Swim-Up Method in Male Infertility

Authors

  • Abida Zuhra Jatiningtyas Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Jawa Tengah, Indonesia
  • Dono Indarto Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Jawa Tengah, Indonesia
  • Isna Qadrijati Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Jawa Tengah, Indonesia
  • Yulia Sari Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Jawa Tengah, Indonesia
  • Lunardhi Susanto School of Pharmacy, Universitas Hang Tuah, Surabaya, Jawa Timur, Indonesia
  • Mulyoto Pangestu Education Program in Reproduction and Development, Department Obstetrics and Gynecology, Monash Clinical School, Monash University, Clayton, VIC, Australia

DOI:

https://doi.org/10.3329/bjms.v24i4.85346

Keywords:

Male infertility; Temperature; DNA fragmentation; Sperm motility

Abstract

Background Temperature has a complex impact and is likely to influence the early stages of spermatogenesis. This study aimed to determine the effect of differential temperature in sperm preparation using the swim-up method on total motile sperm count (TMSC) and DNA fragmentation index (DFI) of male infertility.

Methods This experimental study recruited 37 infertile male patients who attended the Sekar Fertility Clinic, Dr. Moewardi General Hospital, in November 2020, and we added more participants in December 2022. Before collecting semen samples, all infertile males signed informed consent. The collected sample is divided into 1 ml for sperm preparation in groups A (27°C) and B (37°C). The Wilcoxon test analyzed the data; p <0.05 was considered significant.

Results The mean TMSC result in Group A was 17.00 ± 13.06 x106 versus the mean TMSC in Group B at 21.19 ± 17.20 x106; p=0.004, indicating a lower TMSC in Group A. The mean DFI result in group A was 13.74 ± 10.54% versus group B at 13.35 ± 11.78%; p=0.261, also lower in group A.

Conclusion Sperm preparation using the swim-up at 27°C significantly lowers TMSC in infertile males compared to 37°C, but the difference in DFI is not significant compared to 37°C.

BJMS, Vol. 24 No. 04 October’25 Page : 1222-1227

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Published

2025-11-02

How to Cite

Jatiningtyas, A. Z., Indarto, D., Qadrijati, I., Sari, Y., Susanto, L., & Pangestu, M. (2025). Lower temperature (27°C) Decreased Total Motile Sperm Count but not DNA Fragmentation Index in Sperm Preparation with Swim-Up Method in Male Infertility. Bangladesh Journal of Medical Science, 24(4), 1222–1227. https://doi.org/10.3329/bjms.v24i4.85346

Issue

Vol. 24 No. 4 (2025)

Section

Original Articles

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Copyright (c) 2025 Abida Zuhra Jatiningtyas, Dono Indarto, Isna Qadrijati, Yulia Sari, Lunardhi Susanto, Mulyoto Pangestu

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