Assessment of a rapid pan-antibody dot test for detection of antibodies against SARS-CoV-2
Keywords:Cross-sectional study; SARS-CoV-2; seroepidemiological study; immunoblotting; herd-immunity; antibodies.
Background: With the drastic spread of COVID-19 and mass mortality of people globally, detection of the progression of this disease has stood out to be a necessity. Hence, we set out to identify the prevalence of COVID-19 antibodies in Bangladesh using the in-house rapid pan-immunoglobulin dot-blot test kit and evaluate the performance of this kit.
Methods: In this cross-sectional study, we tested serum collected between mid-May and mid-June 2020 for COVID-19 antibodies by using the in-house rapid pan-immunoglobulin dot-blot test kit in RTPCR confirmed patients with symptoms for 1-7 days (Group Ia; n =100) and 8-14 days (Group Ib; n = 100); symptomatic RT-PCR negative patients (Group II; n = 100) and convalescent patients (Group III; n = 109) while comparing with pre-pandemic sera samples collected prior two years to December-2019 (Group IV; n = 100).
Results: Our kit detected that almost 70% of the convalescent patients produced antibodies against COVID-19 compared to other groups. However, the group with individuals at the end phase of COVID-19 exhibited the second-highest percentage of seroprevalence (41%). We also observed that though Group II was RT-PCR negative, 20% of them showed COVID-19 antibodies.
Conclusion: With a specificity of 96% in our kit, we can say that our kit will be a potential device for the detection of SARS-CoV-2 antibodies and to understand herd immunity in Bangladesh.
Bangladesh Journal of Medical Science Vol.20(5) 2021 p.131-139
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Copyright (c) 2021 Md Ahsanul Haq, Maha Jamiruddin, Mohib Ullah Khondoker, Md Firoz Ahmed, Shahad Saif Khandker, Tamanna Ali, Mamun Mostafi, Bijon Kumar Sil, Nihad Adnan, Mohd Raeed Jamiruddin
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