Pathogenic potential of Helicobacter pylori strains can explain differences in H. pylori associated diseases rates from Chile and Cuba

  • Ileana González Biomedical Research Labs, Medicine Faculty, Catholic University of Maule, Talca, Chile
  • Lidice González Department of Research and Development, Division of Equipments and Diagnostics, National Centre for Scientific Research, Havana, Cuba
  • Armando Rojas Biomedical Research Labs, Medicine Faculty, Catholic University of Maule, Talca, Chile
  • Boris L Rodríguez Department of Research and Development, Division of Equipments and Diagnostics, National Centre for Scientific Research, Havana, Cuba
  • Jacqueline Romero Biomedical Research Labs, Medicine Faculty, Catholic University of Maule, Talca, Chile
  • Orlando Reyes Department of Research and Development, Division of Equipments and Diagnostics, National Centre for Scientific Research, Havana, Cuba
  • Erik Morales Biomedical Research Labs, Medicine Faculty, Catholic University of Maule, Talca, Chile & Pathology Department, Regional Hospital of Talca, Chile
  • Jordis Alonso Department of Gastroenterology, Medical and Surgery Research Hospital, Havana, Cuba
  • Ramón Perez Castro Biomedical Research Labs, Medicine Faculty, Catholic University of Maule, Talca, Chile
  • Carlos A Sabatier Anatomopathology Department, Medical and Surgery Research Hospital, Havana, Cuba
  • Eliana Valdés Gastroenterology Unit, Regional Hospital of Talca, Chile
  • Lino E Torres Department of Research and Development, Division of Equipments and Diagnostics, National Centre for Scientific Research, Havana, Cuba
  • Cecilia Cofre Gastroenterology Unit, Regional Hospital of Talca, Chile
Keywords: Helicobacter pylori, EPIYA motifs, Gastric cancer, Virulence

Abstract

Background: The prevalence of Helicobacter pylori-related diseases varies geographically and it is partially determined by the virulence of the circulating strains. Cuba and Chile exhibit different gastric cancer rates, on despite of very similar H. pylori infection rates. We determined if differences in the pathogenic potential of H. pylori isolates from Chile and Cuba could explain the disease outcome in each population.

Methods: H. pylori isolates from 78 Chilean and 71 Cuban patients were analyzed using PCR for the presence of cagA, babA2, vacA alleles and the pattern of EPIYA motifs. Results: cagA was detected in 94.9 % of Chilean and 64.7 % of Cuban isolates (P < 0.001) and was significantly associated with duodenal ulcer (DU) in Cuba (P < 0.01) but not in Chile. The presence of cagA with multiple EPIYA-C motifs was 18.2 % higher in Chile than in Cuba (P < 0.05). Also, an association was observed between GU (P ≤ 0.05) and premalignant lesions (P < 0.001) with the multiple EPIYA-C motif status of the strains in Chile, but not in Cuba. The prevalence of vacA s2m2 genotype was predominant in Chile (66.7 %), while in Cuba was prevalent the s1m1 genotype (56.8 %); and the last one was significantly associated with the presence of DU in Cuban patients.

Conclusions: The cagA status and the EPIYA pattern found in Chilean and Cuban H. pylori clinical isolates partially explain the differences in disease prevalence between both countries. The high proportion of vacA s2m2 genotype in Chile was an unexpected result, needing further studies.

Bangladesh Journal of Medical Science Vol.18(3) 2019 p.577-585

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Published
2019-05-30
How to Cite
González, I., González, L., Rojas, A., Rodríguez, B., Romero, J., Reyes, O., Morales, E., Alonso, J., Castro, R., Sabatier, C., Valdés, E., Torres, L., & Cofre, C. (2019). Pathogenic potential of Helicobacter pylori strains can explain differences in H. pylori associated diseases rates from Chile and Cuba. Bangladesh Journal of Medical Science, 18(3), 577-585. https://doi.org/10.3329/bjms.v18i3.41629
Section
Original Articles