Protective Effect of Bixin Isolated from Bixa orellana L. Seeds on UVB-Induced Inflammation and Immunosuppression of the Skin

  • Atina Hussaana Department of Pharmacology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang
  • - Suparmi Department of Bioology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang
  • Hani Afnita Murti Department of Physic, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang
Keywords: Bixa orellana L.; photoprotection; inflammation; immunosuppression; UVB radiation

Abstract

Background: DNA damage caused by excessive ultraviolet B (UVB) radiation on the skin triggers the response to inflammatory and immunosuppression. The bixin from Bixa orellana L. has been proven to be able to inhibit cyclo-oxygenase.

Objective: to verify whether the bixin lotion has the effect to offer protection against inflammation and immunosuppression due to acute UVB irradiation in shaved BALB /c mice.

Methods: Protection against inflammation and immunosuppression, respectively were studied in 4 groups of mice. Each group was treated respectively with topical application of base lotion as a control; bixin lotion doses of 0.5 mg; 2.5 mg and 125 mg, for 10 days prior to and during the UVB irradiation. The Inflammation was induced by UVB irradiation, 360 mJ/cm2 once a day for 3 consecutive days, whereas the immunosuppression was induced by UVB irradiation, 360 mJ/cm2 once a day for 5 consecutive days. The inflammatory response was measured as an increase in middorsal skinfold thickness at the peak response. The immune response was measured as the contact hypersensitivity (CHS) response to oxasolon sensitization.

Results: The results indicated that in concentration range used, bixin lotion significantly decreased the middorsal skinfold thickness at 72 hours after UVB radiation (p <0.05) compared to the control, but there was no significant difference between couples of the dose of bixin. Bixin lotion was also capable to restore the suppression of CHS from 34.22% in the control group to 11.4%; 0.5% and 0% at doses of 0.5; 2.5 and 125 mg respectively (p <0.05).

Conclusion: Bixin lotion has the potential to reduce the inflammatory edema reaction and the suppression of CHS of mice induced by UVB radiation.

Bangladesh Journal of Medical Science Vol.18(1) 2019 p.107-111

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Author Biographies

Atina Hussaana, Department of Pharmacology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang

Department of Pharmacology

- Suparmi, Department of Bioology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang

Department of Bioology

Hani Afnita Murti, Department of Physic, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang

Department of Physic

Published
2018-12-30
How to Cite
Hussaana, A., Suparmi, -, & Murti, H. (2018). Protective Effect of Bixin Isolated from Bixa orellana L. Seeds on UVB-Induced Inflammation and Immunosuppression of the Skin. Bangladesh Journal of Medical Science, 18(1), 107-111. https://doi.org/10.3329/bjms.v18i1.39559
Section
Original Articles