Effects of polysaccharides from Cordyceps sinensis mycelium on physical fatigue in mice

Feng Yan, Yan Zhang, Beibei Wang

Abstract

This study was designed to determine the effects of polysaccharides from Cordyceps sinensis mycelium (CSP) on physical fatigue in mice. The mice were randomly divided into four groups (n = 16 in each group), i.e. control group, low-dose CSP treated group, intermediate-dose CSP treated group and high-dose CSP treated group. The mice in the treated groups received CSP (100, 200 and 400 mg/kg, ig), and the mice in the control group received drinking water ig. After 28 days, a forced swimming test was performed and some biochemical parameters related to fatigue were examined. The present data suggested that CSP could extend the exhaustive swimming time of mice, as well as increase the hepatic glycogen and muscle glycogen levels, and decrease the blood lactic acid and BUN levels. These results indicated that CSP had anti-fatigue effects.

Keywords

Cordyceps sinensis; forced swimming test; Mycelium; Polysaccharide

Full Text:

Download Read

References

Ataka S, Tanaka M, Nozaki S, Mizuma H, Mizuno K, Tahara T, Sugino T, Shirai T, Kajimoto Y, Kuratsune H, Kajimoto O, Watanabe Y. Effects of applephenon and ascorbic acid on physical fatigue. Nutrition 2007; 23: 419-23.

Chen YQ, Hu B, Xu F, Zhang W, Zhou H, Qu LH. Genetic variation of Cordyceps sinensis, a fruit-body-producing entomopathogenic species from different geographical regions in China. FEMS Microbiol Lett. 2004; 230: 153-58.

Ding JF, Li YY, Xu JJ, Su XR, Gao X, Yue FP. Study on effect of jellyfish collagen hydrolysate on anti-fatigue and anti-oxidation. Food Hydrocoll. 2011; 25: 1350-53.

Dong CH, Xie XQ, Wang XL, Zhan Y, Yao YJ. Application of Box-Behnken design in optimisation for polysaccharides extraction from cultured mycelium of Cordyceps sinensis. Food Bioprod Process. 2009; 87: 139-44.

Evans WJ, Lambert CP. Physiological basis of fatigue. Am J Phys Med Rehabil. 2007; 86: S29-46.

Huang LZ, Huang BK, Ye Q, Qin LP. Bioactivity-guided fractionation for anti-fatigue property of Acanthopanax senticosus. J Ethnopharmacol. 2011; 133: 213-39.

Ikeuchi M, Koyama T, Takahashi J, Yazawa K. Effects of astaxanthin supplementation on exercise-induced fatigue in mice. Biol Pharm Bull. 2006; 29: 2106-10.

Ji DB, Ye J, Li CL, Wang YH, Zhao J, Cai SQ. Antiaging effect of Cordyceps sinensis extract. Phytother Res. 2009; 23: 116-22.

Jin G, Kataoka Y, Tanaka M, Mizuma H, Nozaki S, Tahara T, Mizuno K, Yamato M, Watanabe Y. Changes in plasma and tissue amino acid levels in an animal model of complex fatigue. Nutrition 2009; 25: 597-607.

Kuo MC, Chang CY, Cheng TL, Wu MJ. Immunomodulatory effect of exo-polysaccharides from submerged cultured Cordyceps sinensis: Enhancement of cytokine synthesis, CD11b expression, and phagocytosis. Appl Microbiol Biotechnol. 2007; 75: 769-75.

Li SP, Zhang GH, Zeng Q, Huang ZG, Wang YT, Dong TT, Tsim KW. Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine 2005; 13: 428-33.

Tan W, Yu KQ, Liu YY, Ouyang MZ, Yan MH, Luo R, Zhao XS. Anti-fatigue activity of polysaccharides extract from radix Rehmanniae preparata. Int J Biol Macromol. 2012; 50: 59-62.

Tang W, Zhang Y, Gao J, Ding X, Gao S. The anti-fatigue effect of 20(R)-ginsenoside Rg3 in mice by intranasally administration. Biol Pharm Bull. 2008; 31: 2024-27.

Tharakan B, Manyam BV. Botanical therapies in chronic fatigue. Phytother Res. 2006; 20: 91-95.

Wang JJ, Shieh MJ, Kuo SL, Lee CL, Pan TM. Effect of red mold rice on antifatigue and exercise-related changes in lipid peroxidation in endurance exercise. Appl Microbiol Biotechnol. 2006; 70: 247-53.

Wang Y, Wang M, Ling Y, Fan W, Wang Y, Yin H. Structural determination and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps sinensis. Am J Chin Med. 2009; 37: 977-89.

Wu YL, Sun CR, Pan YJ. Studies on isolation and structural features of a polysaccharide from the mycelium of an Chinese edible fungus (Cordyceps sinensis). Carbohydr Polym. 2006; 63: 251-56.

Xu ZX, Wang CF. Effects of Radix puerariae (Ge Gen) Water Extract on Exercise-Induced Fatigue in Mice. J Anim Vet Adv. 2012; 11: 1722-26.

Yu F, Lu S, Yu F, Feng S, McGuire PM, Li R, Wang R. Protective effects of polysaccharide from Euphorbia kansui (Euphorbiaceae) on the swimming exercise-induced oxidative stress in mice. Can J Physiol Pharmacol. 2006; 84: 1071-79.

Yu FR, Liu Y, Cui YZ, Chan EQ, Xie MR, McGuire PP, Yu FH. Effects of a flavonoid extract from Cynomorium songaricum on the swimming endurance of rats. Am J Chin Med. 2010; 38: 65-73.

Zhang J, Yu Y, Zhang Z, Ding Y, Dai X, Li Y. Effect of polysaccharide from cultured Cordyceps sinensis on immune function and anti-oxidation activity of mice exposed to 60Co. Int Immunopharmacol. 2011; 11: 2251-57.

Zhang XL, Ren F, Huang W, Ding RT, Zhou QS, Liu XW. Anti-fatigue activity of extracts of stem bark from Acanthopanax senticosus. Molecules 2010; 16: 28-37.



DOI: http://dx.doi.org/10.3329/bjp.v7i3.11727

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2012 Feng Yan, Yan Zhang, Beibei Wang

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.