Comparison of the efficacy of selected bacterins against Edwardsiella tarda in immunized Japanese eel (Anguilla japonica)

  • MMM Hossain Department of Fisheries and Marine Bioscience, Jessore Science and Technology University, Jessore
  • Kenji Kawai Fish Disease Laboratory, Department of Aquaculture, Faculty of Agriculture, Kochi University, Nankoku shi, Kochi
  • Jim Duston Department of Plant and Animal Science, Aquaculture Centre, Nova Scotia Agricultural College, Truro, Nova Scotia
  • Syunichirou Oshima Fish Disease Laboratory, Department of Aquaculture, Faculty of Agriculture, Kochi University, Nankoku shi, Kochi
Keywords: Edwardsiella tarda, Protective immunity, Antibody titer, Anguilla japonica, Vaccine, Pressure

Abstract

An effective vaccine against Edwardsiella tarda has not been reported in substitution for high concentration of formalin for the prevention of edwardsiellosis disease. In this study, the efficacy of inactivated E. tarda was evaluated and compared by intraperitoneal (IP) injection-immunization or challenge against Japanese eel (Anguilla japonica). Formalin, formalin with heat, citric acid, pressure and electric current were used for inactivation of the bacteria, and the relative percent survival (RPS) values of pressure (600 psi for 5 min) killed cells was determined. PKC-inactivated vaccine showed-89-93 protection that was higher than others. PKC-inactivated vaccine at a concentration of 106 cells/fish was sufficient to induce high protection (RPS>89). Protection of the different-inactivated vaccines was evaluated at different time post immunization, and the peak of protection was observed at 9 days post-challenge. Fish immunized with PKC showed significantly (P<0.05) higher serum and mucus antibody titers elicit both systemic and mucosal adaptive immune responses, and induce specific humoral immune responses in eel. Coincident with higher protection, sera of fish immunized with the PKC vaccine had higher agglutination titers than FKC, FHKC, CAKC and ECKC. All these data strongly suggested that PKC vaccine is an effective strategy to protect eel against edwardsiellosis.

DOI: http://dx.doi.org/10.3329/jbau.v10i2.14929

J. Bangladesh Agril. Univ. 10(2): 355-366, 2012

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Abstract
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Published
2013-05-13
How to Cite
Hossain, M., Kawai, K., Duston, J., & Oshima, S. (2013). Comparison of the efficacy of selected bacterins against Edwardsiella tarda in immunized Japanese eel (Anguilla japonica). Journal of the Bangladesh Agricultural University, 10(2), 355-366. https://doi.org/10.3329/jbau.v10i2.14929
Section
Fisheries