PCR-based survey of vector-borne pathogens in dogs in Dhaka, Bangladesh

  • MH Talukder Department of Parasitology, Bangladesh Agricultural University, Mymensingh
  • A Matsuu Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University, Koyama-Minami 4-101, Tottori
  • A Iguchi Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University, Koyama-Minami 4-101, Tottori
  • BC Roy Department of Parasitology, Bangladesh Agricultural University, Mymensingh
  • N Nishii Department of Pharmacotherapeutics, Faculty of Agriculture, Tottori University, Koyama-Minami 4-101, Tottori
  • Y Hikasa Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University, Koyama-Minami 4-101, Tottori
Keywords: Tick-borne-pathogen, Babesia gibsoni, Mycoplasma haemocanis, Anaplasma sp. AnHl446

Abstract

To identify tick-borne pathogens in blood samples from 68 dogs were examined for Babesia, Anaplasma/Ehrichia, Mycoplasma, and Hepatozoon spp. by using a molecular tool. The detection of DNA was performed using specific primers for partial 16S rRNA or 18S rRNA sequence, and amplicon sequences were analyzed. Our investigation detected Babesia gibsoni (26 dogs) and Anaplasma sp. AnHl446 (2 dogs), Mycoplasma haemocanis (27 dogs), and an unknown Mycoplasma spp were detected (2 dogs), which was most closely related to Candidatus Mycoplasma turicensis, on the basis of phylogenetic analysis. Hepatozoon DNA could not be detected in this study.

DOI: http://dx.doi.org/10.3329/jbau.v10i2.14915

J. Bangladesh Agril. Univ. 10(2): 249-253, 2012

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Abstract
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Published
2013-05-13
How to Cite
Talukder, M., Matsuu, A., Iguchi, A., Roy, B., Nishii, N., & Hikasa, Y. (2013). PCR-based survey of vector-borne pathogens in dogs in Dhaka, Bangladesh. Journal of the Bangladesh Agricultural University, 10(2), 249-254. https://doi.org/10.3329/jbau.v10i2.14915
Section
Livestock