Isolation, characterization, and molecular identification of Saccharomyces strains from reddragon fruit peel (Hylocereus polyrhizus) for potential application in feed fermentation
Keywords:
Saccharomyces cerevisiae; red dragon fruit peel; yeast isolation; ITS sequencing; fermentation performanceAbstract
Objectives: Red dragon fruit peel is a rich niche for indigenous yeasts with potential applications in feed fermentation. This study aimed to isolate, characterize, and molecularly identify Saccharomyces strains associated with red dragon fruit (Hylocereus polyrhizus) peel from An Giang province, Vietnam, and to assess their suitability as starter cultures for animal feed fermentation. Materials and Methods: A total of thirty yeast strains were isolated, of which 11 exhibited growth on YPDA medium supplemented with sodium bisulfite (NaHSO₃), suggesting sulfite tolerance. Morphological and biochemical characteristics were assessed, and ITS-based DNA sequencing was performed for molecular identification. Results: Six strains (CT6a, CT6b, CT6d, CT6g, CT3e, CT4a) showed high and stable biomass production and efficient fermentation performance in red dragon fruit peel extract. Sequence analysis confirmed all six as Saccharomyces cerevisiae with > 99% identity, and phylogenetic analysis further supported their clustering within the S. cerevisiae clade. Conclusions: Red dragon fruit peel represents a promising substrate for fermentation, and the identified native S. cerevisiae strains demonstrate strong potential as starter cultures in biotechnological applications, particularly for animal feed fermentation.
J. Adv. Vet. Anim. Res., 13(1): 87-96, March 2026
10
10
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Nguyen Thi Hanh Chi, Tran Trung Tuan, Pham Duc Tho
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).