Osteopontin (SPP1) expression on gallstone formation in rabbits fed with a lithogenic diet
Keywords:
Osteopontin; gene expression; gallbladder stone; cholelithiasis; oxidative stress; lithogenic dietAbstract
Objective: This research was designed to assess the influence of the administration of a lithogenic diet, hydrogen peroxide, and vitamin AD3E on rabbits’ gallstone formation and to envisage the expression of osteopontin (OPN) in their hepatic tissues. Materials and Methods: Twenty-four healthy local mature rabbits of both genders were divided into four equal groups. At the end of the feeding period, samples of blood were taken from all rabbits after they had fasted overnight to estimate the serum lipid profile. And some of the hepatic tissue has been preserved at −28°C for molecular analysis and gene expression. Results: The gallstones were formed 100% in the GIII and 50% in the GIV. The mRNA OPN expression showed a significant increase in the GIII when compared with other groups. In Groups III and II, the serum levels of total cholesterol, Triglyceride, L-C, low-density lipoprotein-choles, and VLDL-C were significantly increased when compared with GI, while in GIII, the serum level of high-density lipoprotein-cholesterol was significantly decreased when compared with GI. Conclusion: It was concluded that the expression of the mRNA OPN was increased in the hepatic tissue of gallstone-formed rabbits.
J. Adv. Vet. Anim. Res., 10(2): 301–307, June 2023
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Tareek Abdulqadir Abdal, Raed Salim Al-Naemi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
