Molecular detection of pathogenic Leptospira spp. in urban rodents from wet markets in northeast Malaysia

Authors

Keywords:

Leptospires; Malaysia; molecular detection; PCR; rodents; wet market

Abstract

Objective: This short study describes the occurrence of pathogenic Leptospira spp. in two major wet markets in Kota Bharu, Kelantan, Malaysia. Materials and Methods: 30 rodents (20 rats and 10 shrews) were caught in 2 wet markets, and a postmortem was performed to extract both kidneys. Molecular diagnosis via polymerase chain reaction (PCR) was conducted to detect leptospiral DNA using universal and pathogenic Leptospira primers, respectively. Results: The results showed that 20/28 (72%) rat samples were detected positive for Leptospira spp, and all shrews were negative. Further sequencing analysis identified L. interrogans and L. borgpetersenii as the most frequently Leptospirosis species from kidney samples. Conclusions: The presented study here sheds light on the presence of pathogenic leptospires harboring the rat population in both wet markets in Kelantan, which presents a great public health risk to wet market workers and visitors.

J. Adv. Vet. Anim. Res., 9(2): 275–281, June 2022

http://doi.org/10.5455/javar.2022.i593

Downloads

Abstract
16
PDF
7

Downloads

Published

2022-06-26

How to Cite

Kamaruzaman, I. N. A., Mokhtar, M. A. M., Ting, H. W., Yuan, Y. K., Shah, A. W. G., Loong, T. W., … Abu Bakar, L. (2022). Molecular detection of pathogenic Leptospira spp. in urban rodents from wet markets in northeast Malaysia. Journal of Advanced Veterinary and Animal Research, 9(2), 275–281. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/80149

Issue

Vol. 9 No. 2 (2022)

Section

Original Articles

License

Copyright (c) 2022 Intan Noor Aina Kamaruzaman, Muhamad Aiman Mohd Mokhtar, Hong Wei Ting, Yong Kai Yuan, Azim Wafiy Gulam Shah, Tan Wan Loong, Nurshahirah Shaharulnizim, Mohd Farhan Hanif Reduan, Fathin Faahimaah Abdul Hamid, Nur Amalina Noralidin, Nur Athirah Abdul Manaf, Che Wan Salma Che Wan Zalat, Loong Shih-Keng, Simon Clegg, Luqman Abu-Bakar

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).