Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites

Authors

  • Rasha Eltaysh National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan ; Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. https://orcid.org/0000-0002-7977-0117
  • Mohamed Abdo Rizk National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. https://orcid.org/0000-0002-5250-6172
  • Shimaa Abd El Salam El Sayed National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan ; Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. https://orcid.org/0000-0001-9224-5731
  • Khaled Abouelnasr Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. https://orcid.org/0000-0002-7353-9811
  • Abdelnaser Ahmed Abdallah Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
  • Ikuo Igarashi National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. https://orcid.org/0000-0003-4361-3942

Keywords:

Babesia; Theileria; Artemisia herbaalba; in vitro; in vivo; combination therapy

Abstract

Objective: The effect of Artemisia herba-alba methanolic extract monotherapy and combination therapies on the in vitro growth of several Babesia and Theileria parasites in vitro and mice was investigated in this study. Materials and Methods: Fluorescence assay using SYBR Green I stain was used to evaluate the antibabesial efficacy inhibitory of A. herba-alba either in vitro or in vivo. Hematological parameters in the treated mice were analyzed using a Celltac MEK-6450 computerized hematology analyzer. Results: Artemisia herba-alba reduced the growth of Babesia bovis, Babesia bigemina, Babesia divergens, Theileria equi, and Babesia caballi in vitro in a dose-dependent manner. The in vitro inhibitory impact of A. herba-alba on B. divergens and B. caballi cultures was amplified when combined with either diminazene aceturate (DA). In B. microti-infected mice, a combination therapy consisting of A. herba-alba and a low DA dose inhibited B. microti growth significantly (p < 0.05) better than treatment with 25 mg kg−1 DA. Conclusions: These data show that A. herba-alba, when paired with a modest DA dose, could be a promising medicinal plant for babesiosis treatment.

J. Adv. Vet. Anim. Res., 9(2): 267–274, June 2022

http://doi.org/10.5455/javar.2022.i592

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Published

2022-06-26

How to Cite

Eltaysh, R., Rizk, M. A., El Sayed, S. A. E. S., Abouelnasr, K., Abdallah, A. A., & Igarashi, I. (2022). Evaluation of the in vitro and in vivo inhibitory effects of Artemisia herba-alba against the growth of piroplasm parasites. Journal of Advanced Veterinary and Animal Research, 9(2), 267–274. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/80147

Issue

Vol. 9 No. 2 (2022)

Section

Original Articles

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Copyright (c) 2022 Rasha Eltaysh, Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Khaled Abouelnasr, Abdelnaser Ahmed Abdallah, Ikuo Igarashi

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