Isolation and molecular-based identification of bacteria from unhatched leftover eggs of ducks in selected mini-hatcheries of Kishoreganj, Bangladesh

  • Sadia Afrin Punom Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Md Shahidur Rahman Khan Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Shayka Tasnim Pritha Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Jayedul Hassan Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Saifur Rahman Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Md Muket Mahmud Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Md Shafiqul Islam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
Keywords: Bacteria, duck mini-hatchery, PCR, unhatched leftover eggs.

Abstract

Objectives: The study was designed for isolation and identification of the bacteria present in unhatched leftover eggs of duck in selected mini-hatcheries of Kishoreganj, Bangladesh.

Materials and Methods: A total of 54 unhatched discarded eggs were collected as samples from different mini-hatcheries of Tarail and Itna Upazilas of Kishoreganj and aseptically carried to the laboratory in the icebox. Surface washings (n = 54) and inner contents (n = 54) were collected and enriched in Luria–Bertani broth followed by the isolation of pure colonies of different bacte­ria onto eosin methylene blue agar, mannitol salt agar, Salmonella–Shigella agar, and blood agar plates. Identification of the bacterial isolates was done by cultural properties, staining, and bio­chemical tests followed by molecular detection by Polymerase chain reaction.

Results: Of 108 samples, 62 were found positive for Salmonella spp. (76%), 59 for E. coli (54%), 52 for Staphylococcus spp. (48%), and 5 for Clostridium spp. (9%). From the egg surface sam­ples, Staphylococcus spp. were recovered in the highest (67%) followed by Salmonella spp. (59%), E. coli (56%), and Clostridium spp. (9%). From the inner contents of eggs, Salmonella spp. were recovered in the highest (56%), followed by E. coli (53%) and Staphylococcus spp. (30%).

Conclusion: The isolated bacteria might be associated with the decreased hatchability and embryo mortality in the mini-hatcheries of duck.

J. Adv. Vet. Anim. Res., 7(1): 164-169, March 2020

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Abstract
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Published
2020-03-12
How to Cite
Punom, S., Khan, M. S., Pritha, S., Hassan, J., Rahman, S., Mahmud, M. M., & Islam, M. S. (2020). Isolation and molecular-based identification of bacteria from unhatched leftover eggs of ducks in selected mini-hatcheries of Kishoreganj, Bangladesh. Journal of Advanced Veterinary and Animal Research, 7(1), 164-169. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/46381
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Short Communications