Polymorphism of CLPG gene in three sheep breeds grown in the steppe zone of the Russian Federation

  • Ivan Fiodorovich Gorlov Volga Region Research Institute of Manufacture and Processing of Meat and Milk Production, Volgograd 400131, Russian Federation
  • Nadezhda Vasilievna Shirokova Volga Region Research Institute of Manufacture and Processing of Meat and Milk Production, Volgograd 400131, Russian Federation
  • Yuri Anatolievich Kolosov Don State Agrarian University, Rostov on Don Province 346493, Russian Federation
  • Anatoli Yurievich Kolosov Don State Agrarian University, Rostov on Don Province 346493, Russian Federation
  • Lyubov Vladimirovna Getmantseva Federal Science Center for Animal Husbandry named after Academy Member L.K., Moscow Region 142132, Russian Federation
  • Marina Ivanovna Slozhenkina Volga Region Research Institute of Manufacture and Processing of Meat and Milk Production, Volgograd 400131, Russian Federation
  • Natalia Ivanovna Mosolova Volgograd State Technical University, Volgograd 400005, Russian Federation
  • Elena Yurievna Anisimova Volgograd State University, Volgograd 400062, Russian Federation
  • Viktor Vladimirovich Ponomariov Volga Region Research Institute of Manufacture and Processing of Meat and Milk Production, Volgograd 400131, Russian Federation
Keywords: Callipyge; marker-assisted selection; PCR-RFLP; Sheep Breeding; single-nucleotide polymorphism

Abstract

Objective: This study aims to investigate the callipyge gene (CLPG) polymorphism in sheep of Edilbay, Volgograd, and Kalmyk breeds.

Materials and Methods: The analysis was performed by the polymerase chain reaction–restriction fragment length polymorphisms method. The objects of the study were Edilbay fat-tailed sheep (n = 500) at the breeding plant Volgograd-Edilbay (Volgograd region), Volgograd finewool sheep (n = 500) at the breeding plant Romashkovskiy (Volgograd region), and Kalmyk fattailed sheep (n = 500) at the breeding plant Kirovsky (the Republic of Kalmykia, Yashkul rayon). To conduct the research, tissue samples of 1 cm² from sheep of Kalmyk and Edilbay breeds were taken from the auricle.

Results: The allelic CLPG gene variants have been determined and genotypes of representative sampling of the three breeds of livestock grown in the steppe zone of Russia. The presented results of the CLPG gene polymorphism in these sheep breeds grown in Russia were obtained for the first time. The research study has revealed that in terms of the CLPG gene, the Edilbay, Volgograd, and Kalmyk sheep breeds have only a homozygous form.

Conclusion: The results obtained expand the current understanding of the molecular markers that characterize the meat qualities of sheep.

J. Adv. Vet. Anim. Res., 7(1): 51-55, March 2020

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Abstract
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Published
2020-03-12
How to Cite
Gorlov, I., Shirokova, N., Kolosov, Y., Kolosov, A., Getmantseva, L., Slozhenkina, M., Mosolova, N., Anisimova, E., & Ponomariov, V. (2020). Polymorphism of CLPG gene in three sheep breeds grown in the steppe zone of the Russian Federation. Journal of Advanced Veterinary and Animal Research, 7(1), 51-55. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/46363
Section
Short Communications