Characterization of mitochondrial COX-1 gene of Sarcoptes scabiei from rabbits in East Java, Indonesia

  • Nunuk Dyah Retno Lastuti Postgraduate School and Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia
  • Anwar Ma’ruf Postgraduate School and Department of Basic Medical Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia
  • Wiwik Misaco Yuniarti Department of Clinical Sciences, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia
Keywords: COX-1; DNA mitochondrial; rabbit; Sarcoptes scabiei.

Abstract

Objective: The purpose of this study was to characterize the mitochondrial COX-1 gene of Sarcoptes scabiei in rabbits from three districts of Malang, Nganjuk, and Kediri, East Java, Indonesia. The gene was aligned with a DNA isolated from S. scabiei of Chong’qing rabbit (accession number: EU256388.1) to construct a molecular analysis of phylogenetic in S. scabiei COX-1 gene.

Materials and Methods: This study has been verified by the Committee Ethics (Faculty of Veterinary Medicine, Universitas Airlangga). The mites were collected and identified from rabbits that have an indication of scabies infection. DNA was extracted with QIAamp DNA mini kit and polymerase chain reaction (PCR) analysis was done. The PCR products were purified with the pro­tocol of the BigDye XTerminator™ Purification Kit (Thermo Scientific) and were double-sequenced with the forward and reverse PCR primers of ABI PRISM 310 Genetic Analyzer. The sequence prod­uct was confirmed with Clone Manager Professional 9 (Sci-Ed Software) and the Neighbor-Joining method was done with MEGA6 to build a phylogenetic tree.

Results: The target product of DNA amplification in this PCR was around 290-bp. The amplicon was visualized in 2% of agarose gel electrophoresis. The homology analysis of these sequences showed that it had more than 99% similarity.

Conclusion: COX-1 gene sequences of S. scabiei from rabbits in Malang, Nganjuk, and Kediri were very similar to COX-1 gene sequences in S. scabiei acquired from several hosts according to NCBI data.

J. Adv. Vet. Anim. Res., 6(4): 445-450, December 2019

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Abstract
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Published
2019-11-18
How to Cite
Lastuti, N. D., Ma’ruf, A., & Yuniarti, W. (2019). Characterization of mitochondrial COX-1 gene of Sarcoptes scabiei from rabbits in East Java, Indonesia. Journal of Advanced Veterinary and Animal Research, 6(4), 445-450. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/44112
Section
Short Communications