Antibacterial efficacy of ethanolic extract of Camellia sinensis and Azadirachta indica leaves on methicillin-resistant Staphylococcus aureus and shiga-toxigenic Escherichia coli

  • Md Asief Hossain Zihadi Department of Pharmacology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Marzia Rahman Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Sudipta Talukder Department of Medicine, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Md Mehedi Hasan Department of Medicine, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
  • Samsun Nahar Department of Physiology, Holy Family Red Crescent Medical College, Dhaka, Bangladesh
  • Mahmudul Hasan Sikder Department of Pharmacology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
Keywords: MRSA; STEC; MIC; green tea; neem; multi-drug resistant

Abstract

Objective: This study aims at investigating the antibacterial potential of ethanolic extract of Camellia sinensis (common name: Green tea) and Azadirachta indica (common name: Neem) leaves on methicillin-resistant Staphylococcus aureus (MRSA) and shiga-toxigenic Escherichia coli (STEC).

Materials and Methods: Fresh leaves were processed and extracted by 99% ethanol and recon­stituted with 50% ethanol before testing. Disk diffusion and broth microdilution methods were used to determine zone diameter of inhibition (ZDI) and minimum inhibitory concentration (MIC), respectively. Nutrient agar plate was used to estimate the minimum bactericidal concentration (MBC).

Results: Maximum ZDI value was observed for green tea against MRSA (7.5 mm) and minimum for neem (4.9 mm). Moreover, the highest ZDI against STEC was also for green tea and the com­bination of green tea and neem (4.5 mm). The MIC values of green tea extract were 15.625 and 31.25 mg/ml against MRSA and STEC, respectively, whereas the MIC of neem was 31.25 and 125 mg/ml, respectively. The combination had similar MIC (46.87 mg/ml) against both organ­isms. Green tea showed the lowest MBC values, 31.25 and 62.5 mg/ml, against MRSA and STEC, respectively. However, MBC of neem and the combination against MRSA and STEC were found >250 mg/ml, >500 mg/ml and 93.75 mg/ml, >375 mg/ml, respectively.

Conclusion: Green tea and neem leaves showed good antimicrobial effects and can be used to explore novel antimicrobial compounds against MRSA and STEC.

J. Adv. Vet. Anim. Res., 6(2): 247-252, June 2019

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Published
2019-06-13
How to Cite
Zihadi, M. A., Rahman, M., Talukder, S., Hasan, M. M., Nahar, S., & Sikder, M. H. (2019). Antibacterial efficacy of ethanolic extract of Camellia sinensis and Azadirachta indica leaves on methicillin-resistant Staphylococcus aureus and shiga-toxigenic Escherichia coli. Journal of Advanced Veterinary and Animal Research, 6(2), 247-252. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/41769
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Original Articles