Anti-trypanosomal activity of crude root extract of Leptadenia hastata (Pers) decne in Wistar rats infected with Trypanosoma brucei brucei and associated hematological changes

  • Samson Anjikwi Malgwi Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria
  • Mohammed Kyari Zango Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria
  • Albert Wulari Mbaya Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria
  • Gamgong Dennis Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria
  • Falmata Kyari Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria
  • Kyari Abba Sanda Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria
  • Stephen Bitrus Balami Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria
  • Abwari David Bwala Department of Biological Science, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Keywords: Anti-trypanosomal activity; in-vivo; Leptadenia hastata; pack cell volume

Abstract

Objective: An in vivo study was carried out to evaluate the possible anti-trypanosomal activity of Leptadenia hastata crude root extract with also its associated hematological changes particularly the packed cell volume (PCV) in experimental Trypanosoma brucei brucei infection using Wistar rats.

Materials and Methods: Thirty Wistar rats comprising of both males and females were categorized into six separate groups starting from A to F. Wistar placed in Group A and Group B were inoculated with T. brucei brucei and administered crude root extract of L. hastata at 100 and 200 mg/kg, respectively, as the treatment. Group C was infected with the parasite but untreated, while Group D was not infected with the parasite and was not treated. The remaining Groups E and F were inoculated with the parasite using diminazene diaceturate at 3.5 and 7.0 mg/kg, respectively. The extract was administered enterally when parasitemia was detected. Standard laboratory techniques were employed to determine parasitemia and PCV after collection of blood samples every 2 days via the tail vein.

Results: Infected Groups (A, B, C, E, and F) showed a pre-patent period 2 days post infection (P.I) with mean parasitic counts of 3.93 ± 2.38, 2.46 ± 2.20, 0.67 ± 0.77, 4.60 ± 4.45, and 1.53 ± 1.44, respectively, which continued unabated in groups treated with the extract.The pack cell volume did not decline significantly in the in Groups A and B. Acute toxicity study revealed the absence of any clinical or behavioral changes suggesting toxicity.

Conclusion: There was no effect on parasitemia of Wistar rats infected with the parasite after adminis­tration of 100 and 200 mg, respectively, using the extract as the treatment. PCV of the groups infected remained fairly constant with the control groups throughout the study with the extract being non-toxic.

J. Adv. Vet. Anim. Res., 6(2): 241-246, June 2019

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Published
2019-06-13
How to Cite
Malgwi, S., Zango, M. K., Mbaya, A., Dennis, G., Kyari, F., Sanda, K., Balami, S., & Bwala, A. (2019). Anti-trypanosomal activity of crude root extract of Leptadenia hastata (Pers) decne in Wistar rats infected with Trypanosoma brucei brucei and associated hematological changes. Journal of Advanced Veterinary and Animal Research, 6(2), 241-246. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/41767
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Original Articles