Exploration of anthelmintic activity of Cassia spp. extracts on gastrointestinal nematodes of sheep

  • Sri Wahyuni Faculty of Animal and Agricultural Sciences, University of Diponegoro (UNDIP), Jalan Prof. Soedarto, UNDIP Tembalang Campus, Semarang 50275, Indonesia
  • Sunarso Sunarso Faculty of Animal and Agricultural Sciences, University of Diponegoro (UNDIP), Jalan Prof. Soedarto, UNDIP Tembalang Campus, Semarang 50275, Indonesia
  • Bambang Waluyo Hadi Eko Prasetiyono Faculty of Animal and Agricultural Sciences, University of Diponegoro (UNDIP), Jalan Prof. Soedarto, UNDIP Tembalang Campus, Semarang 50275, Indonesia
  • Fadjar Satrija Faculty of Veterinary Medicine, IPB University, Jalan Agatis IPB Dramaga Campus, Bogor 16680, Indonesia
Keywords: Anthelmintic effect; C. siamea; C. fistula; C. surattensis; C. spectabilis; H. contortus

Abstract

Objective: This study aimed to explore the phytochemical constituents and anthelmintic activities of four Cassia spp. leaves against Haemonchus contortus.

Materials and Methods: The extracts were prepared from four species of Cassia spp. (C. siamea, C. fistula, C. surattensis, and C. spectabilis). Phytochemical screening of the extract was done based on the Harborne method. Evaluation of the anthelmintic activities against H. contortus was done in vitro using infective larvae (L3) migration inhibition assay (LMIA). Measurement of larvae migrating was conducted through a nylon filter with a pore size of 20 μm. The doses of Cassia spp. extract implemented were 25, 50, 100, and 200 mg/ml.

Results: Tannins, alkaloids, phenol hydroquinone, flavonoids, steroids, triterpenoids, and sapo­nins were present in all the extracts, whereas alkaloids were absent in C. fistula. No triterpenoids were found in C. surattensis and C. spectabilis. Movement of H. contortus larvae was significantly inhibited after exposure to Cassia extracts at various dosage levels (p < 0.05). The test results using LMIA on L3H. contortus showed the lowest inhibition in the negative control. Among the species of Cassia, the C. surattensis (at 200 mg/ml) showed the highest (p < 0.05) inhibition level on the larvae. The latter result corresponded to the effect of albendazole.

Conclusion: Compared to other Cassia spp., C. surattensis exhibited the highest inhibition against L3 H. contortus. However, the inhibition effect of C. surattensis was still lower as compared to albendazole.

J. Adv. Vet. Anim. Res., 6(2): 236-240, June 2019

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Abstract
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Published
2019-06-13
How to Cite
Wahyuni, S., Sunarso, S., Prasetiyono, B. W., & Satrija, F. (2019). Exploration of anthelmintic activity of Cassia spp. extracts on gastrointestinal nematodes of sheep. Journal of Advanced Veterinary and Animal Research, 6(2), 236-240. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/41766
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Short Communications