Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs

  • Khaleel Ibrahem Jawasreh Department of Animal Science, Faculty of Agriculture, Jordan University of Science and Technology, Irbid, Jordan
  • Zuhair Bani Ismail Department of Veterinary Clinical Science, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan
Keywords: Awassi sheep; calpastatin gene polymorphism; hematology; serum biochemistry; stress

Abstract

Objective: This study was conducted to investigate the effect of MspI polymorphism in the calpastatin (CAST) gene on hematology and selected serum biochemical parameters in Awassi lambs.

Materials and Methods: CAST genotypes of 31 Awassi lambs were determined using polymerase chain reaction—restricted fragment length polymorphism method. Hematology, serum biochem­ical analyses, serum levels of triiodothyronine, thyroxine, and cortisol were determined using rou­tine laboratory procedures.

Results: Two CAST genotypes were detected with frequencies of 0.65 and 0.35 for MN (three major bands of 622, 336, and 268 bp) and NN (two major bands of 336 and 268 bp), respectively. Allele frequencies were 0.49 and 0.51 for M and N alleles, respectively. Animals with MN MspI CAST genotype had significantly (p < 0.05) higher neutrophil percentage and neutrophil to lymphocyte ratio but, significantly (p < 0.05) lower lymphocyte percentage and neutrophil to lymphocyte ratio than NN MspI CAST genotype. Serum T3 and cortisol concentrations were significantly (p < 0.05) higher in MN MspI CAST genotype than the NN MspI CAST genotype.

Conclusion: Results of this study indicate that CAST gene heterozygous individuals are healthier than homozygous individual, which may explain the superiority of the CAST gene heterozygous animals in growth performance.

J. Adv. Vet. Anim. Res., 6(2): 193-196, June 2019

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Published
2019-06-13
How to Cite
Jawasreh, K., & Ismail, Z. (2019). Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs. Journal of Advanced Veterinary and Animal Research, 6(2), 193-196. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/41758
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Short Communications