Antibody against synthetic peptide of prolactin-inducible protein homologue precursor (PIP-HP) of Bali Cattle (Bos javanicus) saliva as a potential biomarker for immunoassay development

  • Djoko Kisworo
  • Sulaiman Ngongu Depamede
Keywords: Amino acid, Bactericidal, E. coli, Innate immunity, Lateral flow assay, S. aureus

Abstract

Objective: The present study aimed to produce and analyze antibody against a synthetic amino acid sequence fragment of PIP-HP of Bali cattle saliva.

Materials and Methods: The synthetic amino acid sequence of the PIP-HP (VIRELGICPDDWAVIPIKANRF) was developed, conjugated to bovine serum albumin and was used to immunize Indonesian local rabbits. Positive sera that specific against the PIP-HP were pooled and purified sequentially by implying ammonium sulfate precipitation and protein A affinity methods. Purified antibody was then employed to analyze of PIP-HP in the ruminants’ saliva by means of westernblot assays.

Results: A polyclonal antibody specific to asynthetic amino acid sequence fragment of PIP-HP of Bali cattle saliva was successfully produced. Our results show that the antibody potentially to be used to develop an immuno-diagnostic kit. Furthermore, the antibody was also able to inhibit the growth of both Escherichia coli and Staphylococcus aureus cultures significantly (P<0.001) at concentration of 0.3 mg/mL.

Conclusions: In this study, antibody against synthetic peptide of PIP-HP (VIRELGICPDDWAVIPIKANRF) of Bali cattle saliva origin has been produced successfully. Further studies need to be carried out since the antibody has potential as biomarker for non-invasive immunoassays development.

http://doi.org/10.5455/javar.2018.e264     

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Author Biographies

Djoko Kisworo

1Faculty of Animal Science, University of Mataram.Jl. Majapahit No. 62 Mataram NTB 83125, Indonesia.

Sulaiman Ngongu Depamede

Faculty of Animal Science, University of Mataram.Jl. Majapahit No. 62 Mataram NTB 83125, Indonesia.

Published
2018-06-28
How to Cite
Kisworo, D., & Depamede, S. N. (2018). Antibody against synthetic peptide of prolactin-inducible protein homologue precursor (PIP-HP) of Bali Cattle (Bos javanicus) saliva as a potential biomarker for immunoassay development. Journal of Advanced Veterinary and Animal Research, 5(2), 182-187. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/37164
Section
Original Articles