Phytochemical and antimicrobial profile analysis of Parkia biglobosa against methicillin-resistant Staphylococcus aureus

  • Solomon Jauro
  • Mustapha Bala Abubakar
  • Yaqub Ahmed Geidam
  • Mohammed Yusuf Zanna
  • Iliya Dauda Kwoji
  • Isa Adamu Gulani
  • Iliya Ibrahim
Keywords: Antimicrobial, MRSA, Phytochemical

Abstract

Objective: The study was carried out to analyze the various phytochemical properties and to determine the in-vitro antimicrobial activity of Parkia biglobosa methanolic leaf extract against Methicillin-Resistant Staphylococcus aureus (MRSA).

Materials and methods: The phytochemical screening and Thin Layer Chromatography (TLC) of the methanol extract were conducted using standard methods. The antimicrobial activity of the P. biglobosa methanolic leaf extract on sheep and human MRSA isolates was investigated by disc diffusion method. The extract was tested at different concentrations of 100, 200 and 400 mg/mL and norfloxacin (10 µg) as control. The MIC/MBC were determined by serial dilution method in broth media.

Results: the phytochemical analysis showed the presence of alkaloid, saponins, tannins, cardiac glycosides, flavonoids and terpenoids while TLC result revealed 5 spots with retention factor (Rf) values of 0.14, 0.34, 0.42, 0.56 and 0.66 depicting different component using methanol and chloroform at the ratio of 4:1 as solvent system. The diameter of inhibition zone (DIZ) of the extract on MRSA isolates cultured on agar plate revealed a dose dependent response. The extract had the highest DIZ average replicate value of 20.66±1.76 against human MRSA isolates and 20.66±1.20 was recorded as highest DIZ on the sheep MRSA isolates. Norfloxacin had the highest DIZ of 20.33±0.57 on sheep MRSA isolates and 20.33±1.52 DIZ against human isolates. MIC/MBC revealed the highest values of 6.25 mg/mL/12.5 mg/mL for both sheep and human MRSA isolates.

Conclusion: The susceptibility test of crude methanol extract of P. biglobosa leaf has revealed activity against MRSA. Thereby, attesting to the folkloric claim of using P. biglobosa for ethno-therapeutic purposes against infectious diseases in Nigeria and other places.

http://doi.org/10.5455/javar.2018.e263

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Author Biographies

Solomon Jauro

Department of Veterinary Microbiology, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.

Mustapha Bala Abubakar
Department of Veterinary Microbiology, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.
Yaqub Ahmed Geidam

Department of Veterinary Medicine, University of Maiduguri, PMB 1069 Maiduguri, Borno State, Nigeria.

Mohammed Yusuf Zanna
Department of Veterinary Microbiology, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.
Iliya Dauda Kwoji
Department of Veterinary Microbiology, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.
Isa Adamu Gulani
Department of Veterinary Medicine, University of Maiduguri, PMB 1069 Maiduguri, Borno State, Nigeria.
Iliya Ibrahim

Department of Pharmaceutical Chemistry, University of Maiduguri, PMB 1069 Maiduguri, Borno State, Nigeria.

Published
2018-06-28
How to Cite
Jauro, S., Abubakar, M. B., Geidam, Y. A., Zanna, M. Y., Kwoji, I. D., Gulani, I. A., & Ibrahim, I. (2018). Phytochemical and antimicrobial profile analysis of Parkia biglobosa against methicillin-resistant Staphylococcus aureus. Journal of Advanced Veterinary and Animal Research, 5(2), 173-181. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/37163
Section
Original Articles