Remedy of contamination of multidrug resistant Salmonella and Escherichia coli from betel leaves (Piper betle) keeping them fresh for long time

  • Tazrin Kamal
  • K. H. M. Nazmul Hussain Nazir
  • Md. Shafiullah Parvej
  • Md. Tanvir Rahman
  • Marzia Rahman
  • Mohammad Ferdousur Rahman Khan
  • Wahedul Karim Ansari
  • Md Mostakin Ahamed
  • Sultan Ahmed
  • Md. Liakot Hossen
  • Sayedun Nahar Panna
  • Md. Bahanur Rahman
Keywords: Betel leaves, Contamination, Escherichia coli, Salmonella, Treatment

Abstract

Objective: The present study was carried out to identify the associated Salmonella and Escherichia coli in betel leaves (Piper betle), and to develop an effective method to remove those microbes.

Materials and methods: Betel leaves were collected from local and whole sale markets, and borouj (cultivation place). Salmonella and E. coli were isolated and identified by cultural, morphological, and biochemical tests followed by confirmation by polymerase chain reaction (PCR) targeting the genus specific 16S rRNA genes. Antibiogram of the isolated bacteria was performed by disc diffusion method. Different concentrations of Salmosan-A Soln were used to remediate the contaminating bacteria keeping the quality of betel leaves for longer periods.

Results: Total Salmonella counts in the betel leaves were 3.9×105, 4.9×106, 3.5×104, 1.1×103 and 1.5×103 CFU/mL, while E. coli counts were 5.5×107, 6.3×107, 4.4×105, 3.3×103 and 3.1×103 CFU/mL in the betel leaves collected from K.R. market, Kewatkhali Bazaar, whole sale market, borouj in Kushtia and borouj in Natore, respectively. Antibiogram study revealed that the isolated bacteria were sensitive to doxycyclline, ciprofloxacin, chloramphenicol and cefotaxime. Application of 0.3% Salmosan-A Soln was found to be the most effective and suitable, where <1 CFU/mL in the betel leaves could be detected after treatment for 10 min.

Conclusion: Results of this study indicated that treatment with 0.3% Salmosan-A Soln for 10 min is capable of removing Salmonella and E. coli from betel leaves keeping the treated leaves fresh up to 7 days of post-treatment. Method developed in this study can be adapted to reduce bacterial contamination of betel leaves more effectively in Bangladesh and other betel leaf exporting countries.

http://doi.org/10.5455/javar.2018.e250     

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Author Biographies

Tazrin Kamal

Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

K. H. M. Nazmul Hussain Nazir
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md. Shafiullah Parvej
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md. Tanvir Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Marzia Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Mohammad Ferdousur Rahman Khan
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Wahedul Karim Ansari
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md Mostakin Ahamed
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Sultan Ahmed
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md. Liakot Hossen
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Sayedun Nahar Panna
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md. Bahanur Rahman
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Published
2018-04-04
How to Cite
Kamal, T., Nazir, K. H. M. N. H., Parvej, M. S., Rahman, M. T., Rahman, M., Khan, M. F. R., Ansari, W. K., Ahamed, M. M., Ahmed, S., Hossen, M. L., Panna, S. N., & Rahman, M. B. (2018). Remedy of contamination of multidrug resistant Salmonella and Escherichia coli from betel leaves (Piper betle) keeping them fresh for long time. Journal of Advanced Veterinary and Animal Research, 5(1), 73-80. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/36139
Section
Original Articles