Hematology of layers chickens vaccinated with fowl cholera vaccine and experimentally inoculated with virulent Pasteurella multocida serotypes in Zaria, Nigeria

  • Yusuf Madaki Lekko
  • Aliyu Mohammed Wakawa
  • Paul Ayuba Abdu
  • Moshood Abiola Raji
  • Paul Habila Mamman
  • Yusuf Audu
  • Saraya Yaro Balami
  • Kingsly Uwakwe Ezema
  • Isaac John Omeh
  • Peter Anjili Mishelia
Keywords: Fowl cholera, P. multocida, Layers, Serotype, PCV, HB, ALT, AST, ALP

Abstract

Objective: The objective of this study was to evaluate some hematological parameters in commercial layers inoculated with two virulent Pasteurella multocida serotypes.

Materials and Methods: A total of 84 twenty-week-old black Harco layers were randomly assigned to seven groups (A, B, C, D, E, F and G) with 12 birds per group. 1mLof live attenuated fowl cholera (FC) vaccine was administered subcutaneously at 24 weeks of age to groups A and B, emulsified inactivated (killed) FC vaccine was administered dosed at 0.5 mL per bird subcutaneously at 24 weeks of age to groups C and D, groups E and F were not vaccinated, while group G served as control. Groups A, C and E were inoculated with P. multocida serotype A:1 and groups B, D and F were inoculated with P. multocida serotype A:3. Using McFarland Standard, each bird received a dose of 0.5 mL (0.1 mL intranasally and 0.4 mL intramuscularly) containing 4.5 x 108 cfu/bird.

Results: For PCV (P?0.2692 and P?0.7643) and HB (P?0.2806 and P?0.7266) on day 2 and 10 post inoculation, there was no significant difference between the vaccinated, non-vaccinated groups and control group G. However, there was a highly significant difference P?0.05 in the mean concentrations of ALP between the control group G (67.67±1.453 u/l) vaccinated groups A (80.33±4.98 u/l), B (81.33±2.60 u/l), C (75±6.35 u/l), and D (84±5.132 u/l) and unvaccinated groups E (104±1.528 u/l ), and F (78 ±3.512 u/l) post inoculation.

Conclusion The PCV significantly decrease P?0.05 in layers vaccinated and inoculated with   P.  multocida but  increase in unvaccinated  layers   inoculated P.  multocida.  The mean serum ALP concentration significantly increase P?0.05 in unvaccinated layers inoculated with P. multocida when compared to layers vaccinated and inoculated with P.  multocida.  

http://doi.org/10.5455/javar.2017.d216                      

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Author Biographies

Yusuf Madaki Lekko

Departments of  Veterinary Medicine , Faculty of Veterinary Medicine, University of  Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.

Aliyu Mohammed Wakawa

Departments of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.

Paul Ayuba Abdu
Departments of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
Moshood Abiola Raji

Departments of Veterinary Microbiology, Ahmadu Bello University, Zaria, Nigeria.

Paul Habila Mamman
Departments of Veterinary Microbiology, Ahmadu Bello University, Zaria, Nigeria.
Yusuf Audu

Departments of  Veterinary Medicine , Faculty of Veterinary Medicine, University of  Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.

Saraya Yaro Balami

Veterinary Teaching Hospital, Faculty of  Veterinary Medicine, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.

Kingsly Uwakwe Ezema
Veterinary Teaching Hospital, Faculty of  Veterinary Medicine, University of Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.
Isaac John Omeh

Departments of  Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of  Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.

Peter Anjili Mishelia
Departments of  Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of  Maiduguri, PMB 1069, Maiduguri, Borno State, Nigeria.
Published
2018-01-04
How to Cite
Lekko, Y. M., Wakawa, A. M., Abdu, P. A., Raji, M. A., Mamman, P. H., Audu, Y., Balami, S. Y., Ezema, K. U., Omeh, I. J., & Mishelia, P. A. (2018). Hematology of layers chickens vaccinated with fowl cholera vaccine and experimentally inoculated with virulent Pasteurella multocida serotypes in Zaria, Nigeria. Journal of Advanced Veterinary and Animal Research, 4(3), 234-240. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/34292
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Original Articles