Seasonal variations of cauda epididymal spermatozoa of bucks

  • Dilip Kumar Swain College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh
  • Sarvajeet Yadav College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh
  • Vijay Pandey College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh
Keywords: Cauda epididymis, Comet assay, DNA integrity, HOST, Season, Spermatozoa attributes

Abstract

Objective: The study was conducted to evaluate the influence of season on cauda epididymal spermatozoa isolated from bucks.

Materials and methods: Testes of 30 mature bucks were collected from local slaughter house, and were processed for the retrieval of cauda epididymal spermatozoa for evaluation. Testes were collected in three seasons (winter, summer and rainy), and each season was having 10 pairs of testicles. Recovered spermatozoa from the cauda epididymis were processed immediately for evaluation of semen attributes (Spermatozoa motility, viability, plasma membrane integrity, acrosomal status and DNA integrity).

Results: Physiological effect of season was observed on progressive motility, percent of live spermatozoa, spermatozoal membrane integrity (HOST), acrosomal integrity, capacitation status and DNA integrity. Progressive motility, percent live spermatozoa, HOST positive spermatozoa, were found significantly (P<0.05) high in summer season, whereas, significantly (P<0.05) lower comet positive spermatozoa were found in summer season as compared to rainy and winter. Compromised acrosomal status was seen in winter and rainy seasons as compared to summer.

Conclusion: Compromised acrosome along with plasma membrane and higher percentage of spermatozoa with damaged DNA in cauda spermatozoa were observed during winter and rainy seasons as compared to summer season. Summer season was found to be the most suitable season for collection of cauda epididymal spermatozoa and can effectively be used for assisted reproduction with further investigations of associated mechanisms.

http://doi.org/10.5455/javar.2016.c155

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Author Biographies

Dilip Kumar Swain, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh
Department of Veterinary Physiology
Sarvajeet Yadav, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh
Department of Veterinary Physiology
Vijay Pandey, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan, Mathura-281001, Uttarpradesh
Department of Veterinary Biochemistry
Published
2016-11-03
How to Cite
Swain, D. K., Yadav, S., & Pandey, V. (2016). Seasonal variations of cauda epididymal spermatozoa of bucks. Journal of Advanced Veterinary and Animal Research, 3(3), 263-267. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/30220
Section
Short Communications