PCR-based detection of Schistosoma bovis in cattle in Maiduguri Metropolis and Jere Local Government Areas in Borno State, Nigeria

  • Idris Umar Hambali Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Maiduguri, Borno State, PMB 1069
  • Musa Isiaku Ahmed Department of Veterinary Parasitology, University of Ilorin, Ilorin, Kwara State
  • Nuhu Bala Adamu Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Maiduguri, Borno State, PMB 1069
  • Paul Bokko Department of Veterinary Surgery and Theriogenology, University of Maiduguri, Maiduguri, Borno State, PMB 1069
  • Abdulyekeen Olawale Tijjani Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Maiduguri, Borno State, PMB 1069
  • Albert Wulari Mbaya Department of Veterinary Microbiology & Parasitology, University of Maiduguri, Maiduguri, Borno State, PMB 1069
  • Faez Firdaus Abdullah Jesse Department of Veterinary Clinical Studies, Universiti Putra Malaysia
  • Abdullahi Abubakar Biu Department of Veterinary Microbiology & Parasitology, University of Maiduguri, Maiduguri, Borno State, PMB 1069
Keywords: Cattle, Cattle breeds, ELISA, PCR, Prevalence

Abstract

Objective: To determine the prevalence of Schistosoma infection in cattle in Maiduguri Metropolis (MMC) and Jere Local Government Areas (LGAs) of Borno State, Nigeria.

Materials and Methods: Blood samples (n=200) were collected from cattle consisting of one hundred (100) each from five (5) ward levels each of MMC and Jere LGAs. DNA samples were extracted from the serum samples, analysed and quantified using a Nano-drop machine. The extracted DNA were subjected to polymerase chain reaction (PCR).

Results:  The overall prevalence of Schistosoma  infection was 2% (n=200).  Jere LGA had 3% (n=100) while MMC had 1% (n=100). There was no statistical significant association in prevalence rate in the two LGAs studied (P=0.621) (P>0.05). At the ward levels, Custom Area in Jere LGA had 15%, Jiddari ward in MMC had 5%, and the remaining ward levels had no cases. Of the 103 female and 97 male cattle screened, the prevalence in female was 1(0.97%) and 3(3.09%) in the male. Of the 177 serum samples from above 1year (adult) examined, 4 (2.26%) were positive and none in the young. There was no statistical significant association in prevalence rate among ward levels, sex groups and age groups in the study areas (P=0.621) (P>0.05), (P=0.356) (P>0.05) and (P=1.000) (P>0.05) respectively. Of the eight (8) breeds screened, Kuri had 2.7%, Sokoto Gudali (1.82%), Abore (2%), Red Bororo (2.63%), White Fulani, Porland, Mbala and Wafara recorded no cases. The difference in prevalence rates among the breeds based on the trend of occurrence of Schistosoma  infection were not significantly associated statistically (P=1.000) (P>0.05).

Conclusion: There is a prevalence of Schistosoma  infection in cattle in the two LGAs of Borno state. It is recommended that a system be established to maintain preventive and control programs.

http://doi.org/10.5455/javar.2016.c135

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Author Biographies

Paul Bokko, Department of Veterinary Surgery and Theriogenology, University of Maiduguri, Maiduguri, Borno State, PMB 1069

Abdulyekeen Olawale Tijjani, Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Maiduguri, Borno State, PMB 1069

Albert Wulari Mbaya, Department of Veterinary Microbiology & Parasitology, University of Maiduguri, Maiduguri, Borno State, PMB 1069

Faez Firdaus Abdullah Jesse, Department of Veterinary Clinical Studies, Universiti Putra Malaysia

Abdullahi Abubakar Biu, Department of Veterinary Microbiology & Parasitology, University of Maiduguri, Maiduguri, Borno State, PMB 1069

Published
2016-07-25
How to Cite
Hambali, I. U., Ahmed, M. I., Adamu, N. B., Bokko, P., Tijjani, A. O., Mbaya, A. W., Jesse, F. F. A., & Biu, A. A. (2016). PCR-based detection of Schistosoma bovis in cattle in Maiduguri Metropolis and Jere Local Government Areas in Borno State, Nigeria. Journal of Advanced Veterinary and Animal Research, 3(2), 92-98. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/28858
Section
Original Articles