Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns

  • Md. Bakhtiar Lijon Department of Microbiology and Hygiene, FVS, BAU, Mymensingh
  • Mst. Mousumi Khatun Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202
  • Ariful Islam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202
  • Mst. Minara Khatun Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202
  • Md. Ariful Islam Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202
Keywords: Aeromonas hydrophila, Antibiogram profile, Fresh water prawn, Prevalence, PCR assay

Abstract

This  study  was  undertaken  for  isolation,
identification  and  determination  of  antibiogram
profile of Aeromonas hydrophila in farm raised fresh
water  prawn  (Macrobrachium  rosenbergii)  on  five
commercial  ghers.  Fresh  water  prawns  (n=25)  were
collected from five ghers located at Satkhira, Bagerhat
and  Khulna  districts  of  Bangladesh.  Brain  (n=25),
muscle  (n=25)  and  intestine  (n=25)  samples  were
collected  aseptically  from  fresh  water  prawn  and
inoculated  into  alkaline  peptone  (APW)  water  for
enrichment  at  37 ?   for  8  h.  Enriched  cultured  was
streaked  into  Thiosulfate  Citrate  Bile  Salt  Sucrose
(TCBS)  agar  to  isolate  bacteria.  Bacteria  were
identified  by  cultural,  Gram  staining,  biochemical
properties  and  polymerase  chain  reaction  (PCR)
assay.  The  antibiogram  profiles  of  bacteria  were
investigated  against  5  commonly  used  antibiotics
(Gentamicin,  Cefalexin,  Ampicillin,  Azithromycin
and  Ciprofloxacin)  by  disc  diffusion  method.
Thirteen  A.  hydrophila  isolates  were  identified  and
the  prevalence  of  the  A.  hydrophila  in  fresh  water
prawn  was  17.33%.  All  13  (100%)  isolates  were
sensitive to Ciprofloxacin, Gentamicin, Azithromycin
and resistant to Ampicillin and Cefalexin. The results
of  this  study  indicate  that  farm  raised  fresh  water
prawn  harbor  multidrug  resistant  A.  hydrophila
which  might  causes  public  health  problem  if  enter
into human food chain.

http://dx.doi.org/10.5455/javar.2015.b120      

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Abstract
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Published
2015-12-31
How to Cite
Lijon, M., Khatun, M., Islam, A., Khatun, M., & Islam, M. (2015). Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns. Journal of Advanced Veterinary and Animal Research, 2(4), 469-474. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/26096
Section
Original Articles