Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns
This study was undertaken for isolation,
identification and determination of antibiogram
profile of Aeromonas hydrophila in farm raised fresh
water prawn (Macrobrachium rosenbergii) on five
commercial ghers. Fresh water prawns (n=25) were
collected from five ghers located at Satkhira, Bagerhat
and Khulna districts of Bangladesh. Brain (n=25),
muscle (n=25) and intestine (n=25) samples were
collected aseptically from fresh water prawn and
inoculated into alkaline peptone (APW) water for
enrichment at 37 ? for 8 h. Enriched cultured was
streaked into Thiosulfate Citrate Bile Salt Sucrose
(TCBS) agar to isolate bacteria. Bacteria were
identified by cultural, Gram staining, biochemical
properties and polymerase chain reaction (PCR)
assay. The antibiogram profiles of bacteria were
investigated against 5 commonly used antibiotics
(Gentamicin, Cefalexin, Ampicillin, Azithromycin
and Ciprofloxacin) by disc diffusion method.
Thirteen A. hydrophila isolates were identified and
the prevalence of the A. hydrophila in fresh water
prawn was 17.33%. All 13 (100%) isolates were
sensitive to Ciprofloxacin, Gentamicin, Azithromycin
and resistant to Ampicillin and Cefalexin. The results
of this study indicate that farm raised fresh water
prawn harbor multidrug resistant A. hydrophila
which might causes public health problem if enter
into human food chain.
Copyright (c) 2015 Md. Bakhtiar Lijon, Mst. Mousumi Khatun, Ariful Islam, Mst. Minara Khatun, Md. Ariful Islam
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