First report of Bovine Viral Diarrhea Virus antigen from pneumonic cattle in Sudan

  • Intisar Kamil Saeed Virology Department, Veterinary Research Institute, PO Box 8067, Al Amarat, Khartoum, Sudan; Northern Border University, Faculty of Science and Arts, Rafha
  • Yahia Hassan Ali Virology Department, Veterinary Research Institute, PO Box 8067, Al Amarat, Khartoum, Sudan; Northern Border University, Faculty of Science and Arts, Rafha
  • Khalid Mohammed Taha Atbara Veterinary Research Laboratory, PO Box 121 Atbara, River Nile State
  • Nada ElAmin Mohammed Wad Medani Veterinary Research Laboratory, PO Box 555, Gezira State
  • Yasir Mehdi Nouri ElObied Veterinary Research Laboratory, PO Box 373, North Kordofan State
  • Baraa Ahmed Mohammed Virology Department, Veterinary Research Institute, PO Box 8067, Al Amarat, Khartoum
  • Osama Ishag Mohammed Rabak Veterinary Research Laboratory, PO Box 293 White Nile state
  • Salma Bushra Elmagbool Molecular Biology Department, Veterinary Research Institute, PO Box 8067, Al Amarat, Khartoum
  • Fahad Elghazali Molecular Biology Department, Veterinary Research Institute, PO Box 8067, Al Amarat, Khartoum
Keywords: BVDV, NS2-3 protein, Respiratory infections, Sudan

Abstract

To explore the expected role of Bovine Viral Diarrhea Virus (BVDV) in pneumonia in cattle, cattle lungs (n=242) showing signs of pneumonia were collected from slaughter houses of three different localities located at Northern, Central and Western Sudan during 20102013. The collected samples were tested for the presence of BVDV antigen using Enzyme-Linked Immunosorbent Assay (ELISA), and Fluorescent Antibody Test (FAT). Twenty six (10.7%) out of 242 samples were found to be positive for BVDV. Positive results were seen in all the three studied areas, with the highest prevalence (16.7%; n=4/24) at Gezira State in Central Sudan. BVDV genome could be detected in all ELISA positive samples. The results indicated the existence of BVDV infection in cattle in different areas in Sudan, and its possible association with respiratory infections in cattle. Analysis using BLAST indicated that the sequence was identical to the previously reported BVDV-1 (GenBank accession AF220247.1.); nucleotide A was found in our study at position 9 of our sequence, whereas T was present instead in the reference virus. This is the first report of detecting BVDV antigen, genome, and its sequence analysis collected from cattle lungs in Sudan.

 

http://dx.doi.org/10.5455/javar.2015.b67   


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Abstract
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Published
2015-05-20
How to Cite
Saeed, I., Ali, Y., Taha, K., Mohammed, N., Nouri, Y., Mohammed, B., Mohammed, O., Elmagbool, S., & Elghazali, F. (2015). First report of Bovine Viral Diarrhea Virus antigen from pneumonic cattle in Sudan. Journal of Advanced Veterinary and Animal Research, 2(2), 153-157. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/23209
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Original Articles