Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture

Authors

  • Fazle Azim Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • MM Rahman Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • Shamsul H Prodhan Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • Saif U Sikdar Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • Nayem Zobayer Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology
  • M Ashrafuzzaman Department of Genetic Engineering & Biotechnology, Shahjalal University of Science & Technology

DOI:

https://doi.org/10.3329/ijarit.v1i1-2.13935

Keywords:

Malta, Citrus sinensis, In vitro, Seed shoot formation, Callus induction

Abstract

The effects of different hormonal concentration on shoot formation and callus induction were studied on BARI Malta-1 (Citrus sinensis). Seeds containing seed coat and without seed coat was treated by Murashige and Skoog (MS) media supplemented with 6-benzyl adenine (BA) and Kinetin (KIN). Removal of seed coat showed an early response for shoot formation. The highest (70%) shoot formation was obtained from seeds without seed coat treated with MS basal media + BA 1.0 mg/l while KIN showed no response for shoot formation in any supplemented concentration. However, in case of callus induction internodes and apical shoot tips were used as explants and 2, 4-dichlorophenoxy acetic acid (2, 4-D) was used as callus inducing hormone. MS basal media supplemented with 2, 4-D, 2.0 mg/l showed highest (68%) callus induction.

DOI: http://dx.doi.org/10.3329/ijarit.v1i1-2.13935

Int. J. Agril. Res. Innov. & Tech. 1 (1&2): 64-68, December, 2011

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Published

2013-02-23

How to Cite

Azim, F., Rahman, M., Prodhan, S. H., Sikdar, S. U., Zobayer, N., & Ashrafuzzaman, M. (2013). Development of Efficient Callus Initiation of Malta (Citrus sinensis) Through Tissue Culture. International Journal of Agricultural Research, Innovation and Technology, 1(1-2), 64–68. https://doi.org/10.3329/ijarit.v1i1-2.13935

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Articles