Molecular authentication of Euphorbia schimperiana Scheele using internal transcribed spacer sequences of nuclear ribosomal DNA

Authors

  • Mesfer M Alqahtani Department of Biological Sciences, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi 11911, Saudi Arabia
  • M Ajmal Ali Department of Botany and Microbiology, College of Science, King Saud University, Riyadh-11451, Saudi Arabia
  • M Oliur Rahman Department of Botany, University of Dhaka, Dhaka 1000, Bangladesh
  • Fahad M Al Hemaid Department of Botany and Microbiology, College of Science, King Saud University, Riyadh-11451, Saudi Arabia
  • Sidanand V Kambhar Post Graduate Department of Botany, KLE Society’s, Basavaprabhu Kore College, Chikodi-591 201, Belagavi, Karnataka, India
  • Joongku Lee Department of Environment and Forest Resources, Chungnam National University, Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea

DOI:

https://doi.org/10.3329/bjpt.v28i1.54212

Keywords:

Molecular signature; Euphorbia schimperiana; ITS; nrDNA; Phylogenetic relationships.

Abstract

The Internal Transcribed Spacers (ITS) sequences of nuclear ribosomal DNA (nrDNA) are commonly used in plant molecular phylogenetics for the molecular based taxonomic identification and DNA barcoding because of shorter length and easy to amplify by using the universal primers, and further has discrimination ability to distinguish the taxon at lower taxonomic level. The present molecular phylogenetic analysis of ITS nrDNA sequences focuses to determine the taxonomic status of an unresolved medicinally important species Euphorbia schimperiana Scheele of the family Euphorbiaceae reported from Saudi Arabia. The combined length of the entire ITS region in E. schimperiana is 644 nucleotides. The study reveals that E. schimperiana shows a close proximity with the members of the subgenus Esula.

Bangladesh J. Plant Taxon. 28(1): 125-130, 2021 (June)

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Published

2021-06-22

How to Cite

Alqahtani, M. M., Ali, M. A., Rahman, M. O., Al Hemaid, F. M., Kambhar, S. V., & Lee, J. (2021). Molecular authentication of Euphorbia schimperiana Scheele using internal transcribed spacer sequences of nuclear ribosomal DNA. Bangladesh Journal of Plant Taxonomy, 28(1), 125–130. https://doi.org/10.3329/bjpt.v28i1.54212

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