Genetic diversity and phenetic relationships of five <i>Trifolium</i> L. species (Fabaceae) by inter simple sequence repeats markers
Five species of Trifolium L. (T. repens L., T. pretense L., T. hybridum L., T. campestre Schreb., and T. dubium Sibth.) were analyzed used to evaluate the genetic diversity and their phenetic relationships using inter-simple sequence repeats (ISSR) markers. Overall, T. pratense exhibited higher variation than other species. 114 amplicons were produced by ISSR markers, of which 77 (67.5%) bands were polymorphic. T. dubium showed the low genetic variation. Total genetic diversity values (HT) varied between 0.333 and 0.487, for an average over all polymorphic loci of 0.282. On a perlocus basis, the proportion of total genetic variation due to differences among species (GST) was 0.380. This indicated that about 38.0% of the total variation was among species. The estimate of gene flow, based on GST, was very low among species of genus Trifolium (Nm = 0.816). An assessment of the proportion of diversity present within species, HPOP/HSP, indicated that about 95.8% the total genetic diversity was within species. T. pratense and T. hybridum were grouped together and this clade was sister with T. repens. Two remainder species with yellow flowers were grouped together. Information on genetic diversity for Trifolium is valued for the management of germplasm and for evolving conservation strategies.
Bangladesh J. Plant Taxon. 23(2): 167-173, 2016 (December)
Copyright (c) 2016 Bangladesh Association of Plant Taxonomists
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright on any research article is transferred in full to Bangladesh Association of Plant Taxonomists upon publication in Bangladesh Journal of Plant Taxonomy. The copyright transfer includes the right to reproduce and distribute the article in any form of reproduction (printing, electronic media or any other form).
Articles in the Bangladesh Journal of Plant Taxonomy are Open Access articles published under the Creative Commons CC BY License Creative Commons Attribution 4.0 International License.
This license permits use, distribution and reproduction in any medium, provided the original work is properly cited. It is author's responsibility to obtain the permission from appropriate authority if figures are reused from a previously published document.