Application of internal transcribed spacer of nuclear ribosomal DNA for identification of <i>Echinops mandavillei</i> Kit Tan

  • Fahad M.A. Al-Hemaid Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451
  • M. Ajmal Ali Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451
  • Joongku Lee International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305 806
  • Gábor Gyulai Institute of Genetics and Biotechnology, St. István University, Gödöllo H-2103
  • Arun K. Pandey Department of Botany, University of Delhi, Delhi 110007
Keywords: Echinops mandavillei, Asteraceae, ITS, nrDNA, Endemic, Saudi Arabia

Abstract

The present study explored the use of internal transcribed spacers (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA (nrDNA) for identification of Echinops mandavillei Kit Tan, an endemic species to Saudi Arabia. The sequence similarity search using Basic Local Alignment Search Tool (BLAST) and phylogenetic analyses of the ITS sequence of E. mandavillei Kit Tan showed high level of sequence similarity (98%) with E. glaberrimus DC. (section Ritropsis). The novel primary sequence and the secondary structure of ITS2 of E. mandavillei could have a potential use for molecular genotyping.

DOI: http://dx.doi.org/10.3329/bjpt.v21i1.19256

Bangladesh J. Plant Taxon. 21(1): 33-42, 2014 (June)

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Abstract
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Published
2014-06-23
How to Cite
Al-Hemaid, F., Ali, M., Lee, J., Gyulai, G., & Pandey, A. (2014). Application of internal transcribed spacer of nuclear ribosomal DNA for identification of <i>Echinops mandavillei</i&gt; Kit Tan. Bangladesh Journal of Plant Taxonomy, 21(1), 33-42. https://doi.org/10.3329/bjpt.v21i1.19256
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