Antidiarrheal activity of aqueous leaf extract of Ceratotheca sesamoides in rats

  • Yakubu M. Toyin Phytomedicine, Toxicology and Reproductive Biochemistry Research Laboratory, Department of Biochemistry, Ilorin
  • Opakunle F. Khadijat Phytomedicine, Toxicology and Reproductive Biochemistry Research Laboratory, Department of Biochemistry, Ilorin
  • Salimon S. Saoban Phytomedicine, Toxicology and Reproductive Biochemistry Research Laboratory, Department of Biochemistry, Ilorin
  • Ajiboye T. Olakunle Antioxidants, Free Radicals and Toxicology Research Laboratory, Department of Chemical Sciences, Fountain University, Osogbo
  • Bamisaye F. Abraham Department of Biochemistry, Joseph Ayo Babalola University, Ikeji-Arakeji, Osun State
  • Quadri A. Luqman Phytomedicine, Toxicology and Reproductive Biochemistry Research Laboratory, Department of Biochemistry, Ilorin
Keywords: Antidiarrhea, Castor oil, Ceratotheca sesamoides, Enteropooling, Gastrointestinal transit, Pedaliaceae


The antidiarrheal effects of the aqueous leaf extract of C. sesamoides at 25, 50 and 100 mg/kg body weight was evaluated in female rats using gastrointestinal transit, diarrhea and enteropooling induced by castor oil models. The extract was positive for alkaloids, saponins, flavonoids and phenolics. The 25 mg/kg body weight of the extract significantly (p<0.05) prolonged the onset time of diarrhea, decreased the fecal parameters (number, water content, fresh weight, total number of wet feaces) with no episode in the animals treated with 50 and 100 mg/kg body weight. The activity of small intestine Na+-K+ ATPase increased (p<0.05) while the nitric oxide, volume and mass of intestinal fluid as well as the distance travelled by the charcoal meal decreased. The patterns of changes were similar to the reference drugs. Overall, the antidiarrheal activity of the aqueous leaf extract of Ceratotheca sesamoides may be due to alkaloids, phenolics, flavonoids and saponins present in the extract.


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Adegoke E, Akinnsanya A, Nagu A. Studies of Nigerian medicinal plants. J West Afr Sci Ass. 1968; 13: 13-39.

Adzu B, Amos S, Amizan MB, Gamaniel K. Evaluation of the antidiarrhoeal effects of Zizyphus spinachristi stem bark in rats. Acta Trop. 2003; 1: 1-5.

Akanji MA, Yakubu MT. alpha-Tocopherol protects against metabisulphite- induced tissue damage in rats. Nig J Biochem Mol Biol. 2000; 15: 179- 83.

Akindele AJ, Adeyemi OO. Evaluation of the antidiarrhoeal activity of Byrsocarpus coccineus. J Ethnopharmacol. 2006; 108: 20-25.

Anne JM, Geboes K. Infectious colitis. Acta Endoscopica. 2002; 32: 2.

Appidi RJ, Yakubu MT, Grierson DS, Afolayan AJ. Antidiarrhoeal activity of aqueous extract of Hermannia incana Cav. leaves in Wistar rats. Meth Findings Clin Exp Pharmacol. 2010; 32: 27-30.

Bewaji CO, Olorunsogo OO, Bababunmi EA. Comparison of the membrane-bound (Ca2+ + Mg2+)-ATPase in erythrocyte ghosts from some mammalian species. Comp Biochem Physiol. 1985; 82B: 117-22.

Brijesh S, Tetali P, Birdi TJ. Study of effect of anti-diarrheal medicinal plants on enteropathogenic Escherichia coli induced interleukin-8 secretion by intestinal epithelial cells. Altern Med Studies. 2011; 1: e16

Brunton LL. Agents for control of gastric acidity and treatment of peptic ulcers. In: The pharmacological basis of therapeutics. Goodman G (ed). 11th ed. New York, McGraw-Hill, 2008, pp 623-52.

Capasso F, Mascolo N, Izzo AA, Gaginella TS. Dissociation of castor oil-induced diarrhea and intestinal mucosal injury in rat: Effect of NG-nitro-l-arginine methyl ester. Br J Pharmacol. 1994; 113: 1127-30.

Casburn-Jones AC, Farthing MJ. Management of infectious diarrhoea. Gut 2004; 53: 296-305.

ETS. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasbourg, European Treaty Series, ETS-123. 2005.

Fasakin K. Proximate composition of bungu (Ceratotheca sesamoides Endl.) leaves and seeds. Biokemistri 2004; 16: 88-92.

Galvez J, Zarzuelo A, Crespo ME, Lorente MD, Ocete MA, Jimenez J. Antidiarrhoeic activity of Euphorbia hirta extract and isolation of an active flavonoid constituent. Planta Med. 1993; 59: 333-36.

Gerald NT, Jules RK, Omer BN, Donatien G. Antidiarrhoeal and antimicrobial activities of Emilia coccinea (Sims) G. Don extracts. J Ethnopharmacol. 2007; 112: 278-83.

Greenbargena NJ, Arwanitakis C., Hurwitz A, Azarnoff DL. In: drug development of gastrointestinal disorders. New York, Chirchill Livingston, 1978, pp 155-56.

Gurgel LA, Silva RM, Santos FA, Martins DTO, Mattos PO, Rao VSN. Studies on the antidiarrhoeal effect of dragons blood from Croton urucarana. Phytother Res. 2001; 15: 319-22.

Havagiray R, Ramesh C, Sadhna K. Study of antidiarrhoeal activity of Calotropis gigantea R.B.R. in experimental animals. J Pharm Pharmaceut Sci. 2004; 7: 70-75.

Marcos LA, DuPont HL. Advances in defining etiology and new therapeutic approaches in acute diarrhea. J Infect. 2007; 55: 385-93.

Mascolo N, Izzo AA, Barbato F, Capasso F. Inhibitors of nitric oxide synthetase prevent castor-oil induced diarrhoea in the rat. Br J Pharmacol. 1993; 108: 861-64.

Mbagwu HOC, Adeyemi OO. Anti-diarrhoeal activity of the aqueous extract of Mezoneuron benthamianum Baill (Caesalpinaceae). J Ethnopharmacol. 2008; 116: 16-20.

Maridass M. Anti diarrhoeal activity of rare orchid Eulophia epidendraea (Retz.) Fisher. Nature Pharmaceut Technol. 2011; 1: 5-10.

Mukherjee PK, Saha K, Murugesan T, Mandal SC, Pal M, Saha BP. Screening of anti-diarrhoeal profile of some plant extracts of a specific region of Wet Bengal, India. J Ethanopharmacol. 1998; 60: 85-89.

Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 1992; 6: 79-95.

Nwinyi FC, Binda L, Ajoku GA, Aniagu SO, Enwerem NM, Irisadipe A, Kubmarawa D, Gamaniel KS. Evaluation of the aqueous extract of Boswellia dalzielii stem bark for antimicrobial activities and gastrointestinal effects. Afr J Biotechnol. 2004; 3: 284-88.

Otshudi AL, Vercruysse A, Foriers A. Contribution to the ethanobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhoea in Lomela area (DRC). J Ethanopharmacol. 2000; 71: 411-23.

Palombo EA. Phytochemicals from traditional medicinal plants used in treatment of diarrhea: Modes of action and effects on intestinal function. Phytother Res. 2006; 20: 717-24.

Perez GS, Perez GC, Zavala MA. A study of the antidiarrhoeal properties of Loesclia Mexicana on mice and rats. Phytomedicine 2005; 12: 670-71.

Pierce NF, Elliot HI, Greenough WB. Effects of prostaglandins, theophylline and cholera exotoxin upon transmucosal water and electrolyte movement in canine jejunum. J Gastroenterol. 1971; 60: 22-32.

Qnais EY, Abdulla FA, AbuGhalyun YY. Antidiarrheal effects of Juniperus phoenicia L. leaves extract in rats. Pakistan J Biol Sci. 2005; 8: 867-71.

Shoba FG. Study of antidiarrhoeal activity of four medicinal plants in castor oil induced diarrhoea. J. Ethnopharmacol. 2001; 76: 73-76.

Sofowora A. Medicinal plants and traditional medicine in Africa. 2nd ed. Ibadan, Nigeria, Spectrum Books Limited,1993, pp 134-56.

Suleiman MM, Dzenda T, Sani CA. Antidiarrhoeal activity of the methanol stem-bark extract of Annona senegalensis Pers. (Annonaceae). J Ethnopharmacol. 2008; 116: 125-30.

Sunil B, Bedi K, Singla A, Johri R. Antidiarrhoeal activity of piperine in mice. Planta Medica. 2001; 67: 284-87.

Tangpu V, Yadav AK. Antidiarrhoea activity of Rhus javanica extract in albino mice. Fitoterapia 2004; 75: 39-44.

Teke GN, Kuiate JR, Ngouateu OB, Gatsing D. Antidiarrhoeal and antimicrobial activities of Emilia coccinea extracts. J Ethnopharmacol. 2007; 112: 278-83.

Venkatesan N, Vadivu T, Sathiya N, Arokya A, Sundararajan R, Sengodan G, Vijaya K, Thandavarayan R, James BP. Anti-diarrhoeal potential of Asparagus racemosus wild root extracts in laboratory animals. J Pharmaceut Sci. 2005; 8: 39-45.

World Health Organization. World Health Report [Internet]. Geneva: Available from: http://whqlib- 2004, 120-5. Accessed on September 10, 2010.

Veiga VF, Zunino L, Calixto JB, Pititucci ML, Pinato AC. Phytochemical and antioedematogenic studies of commercial copaiba oils available in Brazil. Phytother Res. 2001; 15: 476-80.

How to Cite
Toyin, Y., O. Khadijat, S. Saoban, A. Olakunle, B. Abraham, and Q. Luqman. “Antidiarrheal Activity of Aqueous Leaf Extract of Ceratotheca Sesamoides in Rats”. Bangladesh Journal of Pharmacology, Vol. 7, no. 1, Mar. 2012, pp. 14-20, doi:10.3329/bjp.v7i1.9789.
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