Increased DNA damage in blood cells of rat treated with lead as assessed by comet assay

  • Mohammad Arif Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka
  • Yearul Kabir Department of Family Sciences, College for Women, Kuwait University
  • Faizule Hassan Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka
  • T. M. Zaved Waise Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka
  • Md. Ehsanul Hoque Mazumder Department of Pharmacy, Jahangirnagar University, Dhaka
  • Shafiqur Rahman Department of Pharmaceutical Sciences, South Dakota State University
Keywords: DNA damage, Lead toxicity, Oxidative stress

Abstract

A growing body of evidence suggests that oxidative stress is the key player in the pathogenesis of lead-induced toxicity. The present study investigated lead induced oxidative DNA damage, if any in rat blood cells by alkaline comet assay. Lead was administered intraperitoneally to rats at doses of 25, 50 and 100 mg/kg body weight for 5 days consecutively. Blood collected on day six from sacrificed lead-treated rats was used to assess the extent of DNA damage by comet assay which entailed measurement of comet length, olive tail moment, tail DNA (%) and tail length. The results showed that treatment with lead significantly increased DNA damage in a dose-dependent manner. Therefore, our data suggests that lead treatment is associated with oxidative stress-induced DNA damage in rat blood cells which could be used as an early bio-marker of lead-toxicity.

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Author Biographies

Mohammad Arif, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka

Assistant professor

Yearul Kabir, Department of Family Sciences, College for Women, Kuwait University
Assistant Professor

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Published
2008-07-15
How to Cite
Arif, M., Y. Kabir, F. Hassan, T. M. Waise, M. E. Mazumder, and S. Rahman. “Increased DNA Damage in Blood Cells of Rat Treated With Lead As Assessed by Comet Assay”. Bangladesh Journal of Pharmacology, Vol. 3, no. 2, July 2008, pp. 97-01, doi:10.3329/bjp.v3i2.948.
Section
Research Articles