Occurrence of curcuminoids in Curcuma longa: A quality standardization by HPTLC

Authors

  • M. Paramasivam Regional Analytical Laboratory of Medicinal & Aromatic Plants, Department of Agricultural Chemicals, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur-741252, Nadia, W.B
  • Md. Wasim Aktar Regional Analytical Laboratory of Medicinal and Aromatic Plants, Department of Agricultural Chemicals, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur-741252, Nadia, W.B
  • R. Poi Regional Analytical Laboratory of Medicinal and Aromatic Plants, Department of Agricultural Chemicals, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur-741252, Nadia, W.B
  • H. Banerjee Regional Analytical Laboratory of Medicinal and Aromatic Plants, Department of Agricultural Chemicals, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur-741252, Nadia, W.B
  • A. Bandyopadhyay Department of Spices and plantation Crops, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur-741252, Nadia, W.B

DOI:

https://doi.org/10.3329/bjp.v3i2.833

Keywords:

Curcuminoids, Curcuma longa, HPTLC, Quantitative determination, Zingiberaceae

Abstract

A simple high performance thin layer chromatographic (HPTLC) method has been developed for the simultaneous determination of the pharmacologically important active curcuminoids viz. curcumin, demthoxycurcumin and bis-demethoxycurcumin in Curcuma longa L. The assay combines the separation and quantification of the analytes on silica gel 60 GF254 HPTLC plates with visualisation under UV and scanning at 425 nm. Using this technique, the alkaloidal content of different parts of the title plant has been determined.

Downloads

Download data is not yet available.
Abstract
1402
Download
1161 Read
693

References

Ahsan H, Parveen N, Khan NU, Hadi SM. Pro-oxidant, antioxidant and cleavage activities on DNA of curcumin and its derivatives demethoxycurcumin and bis-demethoxycurcumin. Chem Biol Interact. 1999; 121: 161-75.

Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. Turmeric and curcumin: Biological actions and medicinal applications. Curr Sci. 2004; 87: 44-50.

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. New Delhi, CSTR, 1956, p 7.

Govindarajan VS. Turmeric: Chemistry, technology and quality. Crit Rev Food Sci Nutr. 1980; 12: 199-301.

Gupta AP, Gupta MM, Kumar S. Simultaneous determination of curcuminoids in curcuma samples using high performance thin layer chromatography. J Liq Chromatogr Related Technol. 1999; 22: 1561-69.

Kapoor LD. Handbook of Ayurvedic medicinal plants. Boca Raton, FL, CRC Press, 2001, p 216.

Khurana A, Ho CT. High performance liquid chromatographic analysis of curcuminoids and their photo-oxidative decomposition compounds in Curcuma longa L. J Liq Chromatogr. 1988; 11: 2295-304.

Schieffer GW. Pressurized liquid extraction of curcuminoids and curcuminoid degradation products from turmeric (Curcuma longa) with subsequent HPLC assays. J Liq Chromatogr Related Technol. 2002; 25: 3033-44.

Taylor SJ, McDowell IJ. Determination of the curcuminoid pigments in turmeric (Curcuma domestica Val.) by reversed phase high performance liquid chromatography. Chromatographia 1992; 34: 73-77.

Additional Files

Published

2008-05-09

How to Cite

Paramasivam, M., M. W. Aktar, R. Poi, H. Banerjee, and A. Bandyopadhyay. “Occurrence of Curcuminoids in Curcuma Longa: A Quality Standardization by HPTLC”. Bangladesh Journal of Pharmacology, vol. 3, no. 2, May 2008, pp. 55-58, doi:10.3329/bjp.v3i2.833.

Issue

Section

Research Articles